SIZ1-Mediated SUMOylation of ROS1 Enhances Its Stability and Positively Regulates Active DNA Demethylation in Arabidopsis  

作　　者：Xiangfeng Kong Yechun Hong Yi-Feng Hsu Huan Huang Xue Liu Zhe Song Jian-Kang Zhu 

机构地区：[1]Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,People's Republic of China [2]University of Chinese Academy of Sciences,Beijing,People's Republic of China [3]Department of Horticulture and Landscape Architecture,Purdue University,West Lafayette,IN 47907,USA

出　　处：《Molecular Plant》2020年第12期1816-1824,共9页分子植物（英文版）

基　　金：the Chinese Academy of Sciences to J.-K.Z.

摘　　要：The 5-methylcytosine DNA glycosylase/lyase REPRESSOR OF SILENCING 1(ROS1)-mediated active DNA demethylation is critical for shaping the genomic DNA methylation landscape in Arabidopsis.Whether and how the stability of ROS1 may be regulated by post-translational modifications is unknown.Using a methylation-sensitive PCR(CHOP-PCR)-based forward genetic screen forArabidopsis DNA hyper-methyl-ation mutants,we identified the SUMO E3 ligase SIZ1 as a critical regulator of active DNA demethylation.Dysfunction of SIZ1 leads to hyper-methylation at approximately 1000 genomic regions.SIZ1 physically in-teracts with ROS1 and mediates the SUMOylation of ROS1.The SUMOylation of ROS1 is reduced in siz1 mutant plants.Compared with that in wild-type plants,the protein level of ROS1 is significantly decreased,whereas there is an increased level of ROS1 transcripts in siz1 mutant plants.Our results suggest that SIZ1-mediated SUMOylation of ROS1 promotes its stability and positively regulates active DNA demethylation.

关 键 词：SIZ1 SUMOylatoin ROS1 active DNA demethylation ARABIDOPSIS 

分 类 号：Q94[生物学—植物学]