自体、同种异体纯化富血小板血浆治疗兔膝骨关节炎的差异  

The difference between autologous and allogeneic purified platelet-rich plasma in the treatment of rabbit knee osteoarthritis

作　　者：赵露婷 方其林 唐海燕 胥方元 Zhao Luting;Fang Qilin;Tang Haiyan;Xu Fangyuan(The First People’s Hospital of Ziyang,Ziyang 641300,Sichuan Province,China;Zigong First People’s Hospital,Zigong 643000,Sichuan Province,China;The Affiliated Hospital of Southwest Medical University,Luzhou 646000,Sichuan Province,China)

机构地区：[1]资阳市第一人民医院,四川省资阳市641300 [2]自贡市第一人民医院,四川省自贡市643000 [3]西南医科大学附属医院,四川省泸州市646000

出　　处：《中国组织工程研究》2021年第26期4123-4129,共7页Chinese Journal of Tissue Engineering Research

基　　金：四川省教育厅项目(12ZB255),项目负责人:胥方元。

摘　　要：背景:活化后的纯化富血小板血浆可促进软骨细胞的增殖,调控关节内炎症反应,有效治疗膝骨关节炎。目的:对比自体、同种异体纯化富血小板血浆修复膝骨关节炎的效果。方法:取32只3月龄新西兰大耳兔,采用随机数字表法分4组,每组8只:正常组不作任何处理;模型组、自体纯化富血小板血浆组、异体纯化富血小板血浆组通过关节内注射木瓜蛋白酶与L-半胱氨酸混合溶液制备膝骨关节炎模型,造模2周后,自体纯化富血小板血浆组、异体纯化富血小板血浆组分别关节内注射自体纯化富血小板血浆与同种异体纯化富血小板血浆,每2周注射1次,共注射3次。末次注射2周后,进行关节软骨大体观察、组织学观察与免疫组化观察。实验经西南医科大学实验动物管理委员会批准。结果与结论:①大体观察:模型组关节软骨表面粗糙,出现软骨缺损、侵蚀、溃疡,关节骨赘形成,有大量的关节腔积液,自体纯化富血小板血浆组关节软骨损伤及骨赘形成轻于模型组,异体纯化富血小板血浆组关节软骨损伤及骨赘形成与模型组相比无明显改善;②组织学观察:与模型组相比,自体纯化富血小板血浆组软骨结构、基质染色、细胞、潮线等各方面均明显改善(P均<0.05),异体纯化富血小板血浆组软骨结构有一定改善(P<0.05),但细胞排列、基质染色、潮线方面改善不明显;③免疫组化观察:自体纯化富血小板血浆组、异体纯化富血小板血浆组骨形态发生蛋白2与Sox9表达量的高于模型组(P<0.05),自体纯化富血小板血浆组骨形态发生蛋白2表达量高于异体纯化富血小板血浆组(P<0.05);④结果表明,自体、同种异体纯化富血小板血浆均能在一定程度上改善膝骨关节炎软骨细胞的增殖能力,促进软骨细胞修复,但自体纯化富血小板血浆的治疗效果更为明显。BACKGROUND:Purified platelet-rich plasma after activation can promote the proliferation of chondrocytes,regulate inflammation in the joints,and effectively treat knee osteoarthritis.OBJECTIVE:To compare the effects of autologous and allogeneic purified platelet-rich plasma in repairing knee osteoarthritis.METHODS:Thirty-two 3-month-old New Zealand big-eared rabbits were divided into four groups using a random number table method,with eight rabbits in each group:the normal group was treated without any treatment;in the model,autologous,and allogeneic groups,a knee osteoarthritis model was prepared by intra-articular injection of a mixed solution of papain and L-cysteine.After 2 weeks of modeling,the autologous group and allogeneic group were injected intra-articularly with autologous and allogeneic purified platelet-rich plasma,respectively.Platelet-rich plasma was injected once every 2 weeks for a total of three injections.Two weeks after the last injection,gross observation,histological observation and immunohistochemical observation of the articular cartilage were performed.The experiment was approved by the Laboratory Animal Management Committee of Southwest Medical University.RESULTS AND CONCLUSION:General observation:The surface of the articular cartilage of the model group was rough,cartilage defects,erosions,ulcers,joint osteophytes were formed,and there was a large amount of joint cavity effusion.Articular cartilage damage and osteophytes formation in the autologous group were lighter than those in the model group.Compared with the model group,the articular cartilage injury and osteophyte formation in the allogeneic group were not significantly improved.Histological observation:Compared with the model group,the cartilage structure,matrix staining,cells,and tide lines of the autologous group were significantly improved(P<0.05);the cartilage structure of the allogeneic group was improved(P<0.05),but there was no obvious improvement in cell arrangement,matrix staining,and tide line.Immunohistochemical observ

关 键 词：实验 动物 兔 膝骨关节炎 纯化富血小板血浆 骨形态发生蛋白2 SOX9 

分 类 号：R459.9[医药卫生—治疗学] R319[医药卫生—临床医学]