丝裂原活化蛋白激酶信号通路介导的自噬可调节破骨细胞的增殖分化和功能  

Autophagy regulates osteoclast proliferation,differentiation and function through mitogen-activated protein kinase signaling pathway

作　　者：杨羽晨 杨佩佩 黄碧莹 张强[1] Yang Yuchen;Yang Peipei;Huang Biying;Zhang Qiang(Department of Oral and Maxillofacial Surgery,First Affiliated Hospital of Nanchang University,Nanchang 330006,Jiangxi Province,China)

机构地区：[1]南昌大学第一附属医院口腔颌面外科,江西省南昌市330006

出　　处：《中国组织工程研究》2021年第26期4192-4197,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(81260169,81560189),项目负责人:张强。

摘　　要：背景:骨稳态的维持主要依赖于破骨细胞与成骨细胞之间的协同作用,丝裂原活化蛋白激酶介导的自噬对破骨细胞增殖、分化、功能的影响很有可能会破坏骨稳态平衡,从而影响组织工程的骨重建工作。目的:通过目前已有的实验研究和临床资料分析丝裂原活化蛋白激酶介导的自噬对破骨细胞的影响。方法:计算机检索中国知网、万方医学网、PubMed数据库中的相关文献,检索时间从建库截止至2020-06-31,中文检索词为“丝裂原活化蛋白激酶,破骨细胞,自噬”,英文检索词为“mitogen-activated protein kinase,ERK,p38,JNK,osteoclast,autophagy”。结果与结论:①丝裂原活化蛋白激酶既可以单独介导自噬对破骨细胞产生影响,也可以同时激活其他信号通路共同介导自噬;②细胞外信号调节激酶(ERK)通路介导的自噬常伴随着Ca^(2+)的参与,主要影响破骨细胞的分化和功能;③c-Jun氨基末端激酶(JNK)主要通过与Beclin-1的共同作用影响破骨细胞的成熟和分化;④p38激酶自噬具有促进和抑制的双向作用,从而影响破骨细胞的增殖与分化。BACKGROUND:Bone homeostasis is mainly kept relying on the synergistic effect between osteoclasts and osteoblasts.Mitogen-activated protein kinasemediated autophagy exerts some effects on proliferation,differentiation and function of osteoclasts,which is likely to disrupt the bone homeostasis,thus affecting bone reconstruction in tissue engineering.OBJECTIVE:To analyze the effects of mitogen-activated protein kinase-mediated autophagy on osteoclasts based on existing experimental and clinical data.METHODS:The relevant literatures in CNKI,WanFang,and PubMed database were searched by computer.The retrieval time was from inception to June 31,2020.The keywords were“mitogen activated protein kinase,osteoclast,autophagy”in Chinese and“mitogen-activated protein kinase,ERK,P38,JNK,osteoclast,autophagy”in English.RESULTS AND CONCLUSION:Mitogen-activated protein kinase cannot only mediate autophagy and affect osteoclasts,but also activate other signaling pathways to mediate autophagy together.Autophagy mediated by extracellular signal-regulated kinase pathway is often accompanied by Ca2+involvement,which mainly affects osteoclast differentiation and function.c-Jun N-terminal kinase influences osteoclast maturation and differentiation mainly through the co-action of Beclin-1.p38 kinase-mediated autophagy has a two-way effect on promotion and inhibition,thus affecting the proliferation and differentiation of osteoclasts.

关 键 词：自噬 MAPK 破骨细胞 骨稳态 ERK JNK P38激酶 组织工程 综述 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]