基于DNA折纸模板的铁原子阵列构建及其信息加密应用  

Constructions of iron atoms arrays based on DNA origami templates for cryptography applications

作　　者：凡洪剑 李江 王丽华 樊春海 柳华杰[1] Fan Hong-Jian;Li Jiang;Wang Li-Hua;Fan Chun-Hai;Liu Hua-Jie(School of Chemical Science and Engineering,Shanghai Research Institute for Intelligent Autonomous Systems,Key Laboratory of Advanced Civil Engineering Materials of Ministry of Education,Tongji University,Shanghai 200092,China;Shanghai Synchrotron Radiation Facility,Zhangjiang Laboratory,Shanghai Advanced Research Institute,Chinese Academy of Sciences,Shanghai 201800,China;School of Chemistry and Chemical Engineering,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]同济大学化学科学与工程学院,上海自主智能无人系统科学中心,先进土木工程材料教育部重点实验室,上海200092 [2]中国科学院上海高等研究院,张江实验室,上海光源科学中心,上海201800 [3]上海交通大学化学化工学院,上海200240

出　　处：《物理学报》2021年第6期306-313,共8页Acta Physica Sinica

基　　金：国家重点研发计划(批准号:2016YFA0400900,2016YFA0201200);国家自然科学基金(批准号:21722310,21834007,21873071,91953106);中央高校基本科研业务费专项资金资助的课题.

摘　　要：在后摩尔时代,突破原有技术极限,进行原子尺度的精准构筑,是当前的重大科学问题.DNA作为具有原子级精准度的生物大分子,能够进行程序性的分子识别,构筑原子数量与位置均严格确定的自组装结构,因此是进行原子制造的理想平台.本文提出基于DNA自组装折纸结构的精准定位能力,构筑铁原子阵列图案,并应用于对信息的加密.实验结果表明,采用类似“信息预置”的方法,铁原子成功实现在DNA折纸不同位置的高效定位,此方法还极大降低了实验工作量,非常有利于多种不同阵列图案的平行制备.利用所构建的铁原子阵列,本文发展了原子阵列DNA折纸加密技术,将密文编码为二进制并用类似盲文斑点的形式在DNA折纸上以特定图案表示,通过单分子成像手段对密文信息进行了读取,而密钥长度可高达700位以上.作为示例,成功地对普通文本及唐诗《登鹳雀楼》进行了加密,证明了此策略的通用性和实用性.The fabrication of precise arrays of atoms is a key challenge at present.As a kind of biomacromolecule with strict base-pairing and programmable self-assembly ability,DNA is an idea material for directing atom positioning on predefined addresses.Here in this work,we propose the construction of iron atom arrays based on DNA origami templates and illustrate the potential applications in cryptography.First,ferrocene molecule is used as the carrier for iron atom since the cyclopentadienyl groups protect iron from being affected by the external environment.To characterize the iron atom arrays,streptavidins are labelled according to the ferrocene-modified DNA strand through biotin-streptavidin interactions.Based on atomic force microscopy scanning,ferrocene-modified single-stranded DNA sequences prove to be successfully immobilized on predefined positions on DNA origami templates with high yield.Importantly,the address information of iron atoms on origami is pre-embedded on the long scaffold,enabling the workload and cost to be lowered dramatically.In addition,the iron atom arrays can be used as the platform for constructing secure Braille-like patterns with encoded information.The origami assembly and pattern characterizations are defined as encryption process and readout process,respectively.The ciphertext can be finally decoded with the secure key.This method enables the theoretical key size of more than 700 bits to be realized.Encryption and decryption of plain text and a Chinese Tang poem prove the versatility and feasibility of this strategy.

关 键 词：DNA 折纸 自组装 原子阵列 加密 

分 类 号：TN918.4[电子电信—通信与信息系统]