辣木黄酮对糖尿病脑病大鼠认知功能及神经病理指标的影响  

Effects of Moringa flavone on cognitive function and neuropathological indexes in diabetic encephalopathy rats

作　　者：朱建忠[1] 赵灿 乔跃兵[1] 刘媛媛[1] 隋月林[1] Zhu Jianzhong;Zhao Can;Qiao Yuebing;Liu Yuanyuan;Sui Yuelin(Department of Anatomy,Cangzhou Medical College,Cangzhou 061000,Hebei,China)

机构地区：[1]沧州医学高等专科学校解剖教研室,河北沧州061000

出　　处：《中华危重病急救医学》2020年第12期1491-1495,共5页Chinese Critical Care Medicine

基　　金：国家卫健委重点实验室开放性课题(20150002);河北省科技发展计划项目(08276101D-20);河北省沧州市重点研发计划指导项目(204106039)。

摘　　要：目的探讨辣木黄酮对糖尿病脑病(DE)大鼠认知功能障碍及神经病理指标的影响。方法将60只雄性SD大鼠按照随机数字表法分为对照组、模型组、阳性药物组及辣木低、高剂量组,每组10只。高脂高糖饲料持续喂养1周后经腹腔注射链脲霉菌素(STZ)25 mg/kg建立糖尿病大鼠模型,72 h后尾静脉采血,以2次随机血糖均值≥16.67 mmol/L、尿糖持续阳性表明糖尿病模型制备成功;对照组给予常规饲料喂养。辣木低、高剂量组大鼠制模成功后,分别每日灌胃4.0 g/kg和8.0 g/kg辣木提取物(辣木黄酮);阳性药物组每日灌胃0.48 g/kg吡拉西坦,模型组和对照组每日灌胃等量生理盐水,均每日1次,持续给药30 d。进行Morris水迷宫实验以评估大鼠认知功能障碍情况。于末次给药后12 h分离大鼠海马组织,采用免疫组化染色检测晚期糖基化终末产物受体(RAGE)和核转录因子-κB(NF-κB)表达情况;采用酶联免疫吸附试验(ELISA)检测乙酰胆碱酯酶(AChE)、晚期糖基化终末产物(AGE)和乙酰胆碱转移酶(ChAT)含量。结果与对照组相比,模型组大鼠逃避潜伏期、探索距离明显延长,目标象限停留时间明显缩短,脑组织AChE和AGE水平显著升高,ChAT水平显著降低。Morris水迷宫实验显示:与模型组相比,辣木低、高剂量组第3天起大鼠逃避潜伏期(s:35.07±7.21、33.14±5.35比43.09±9.83,均P<0.05)和缩短探索距离(m:8.32±4.23、8.10±4.97比13.02±3.67)均明显缩短(均P<0.05),目标象限停留时间明显延长(s:35.12±3.12、41.53±8.37比23.15±4.89,均P<0.01)。ELISA结果显示,与模型组比较,辣木低、高剂量组脑组织中AChE和AGE水平显著降低〔AChE(U/L):180.22±12.03、142.67±20.56比205.27±25.14,AGE(μg/L):439.10±25.19、428.27±19.14比501.28±21.53,均P<0.05〕,ChAT水平显著升高(U/L:51.95±5.27、53.13±5.04比37.91±5.10,均P<0.01);辣木低、高剂量组组间AChE、AGE和ChAT水平比较差异均无统计学意义。免疫组化Objective To investigate the effect of Moringa flavone on cognitive impairment and neuropathological indexes in diabetic encephalopathy(DE)rats.Methods Sixty male Sprague-Dawley(SD)rats were divided into control group,model group,positive drug group,Moringa low-dose and high-dose groups according to the random number table method,with 10 rats in each group.Diabetic rat model was established by intraperitoneal injection of 25 mg/kg streptozotocin(STZ)after continuous feeding of high fat and high sugar diet for one week.Blood was collected from the tail vein after 72 hours,the mean value of twice random blood glucose was≥16.67 mmol/L,and the continuous positive urine glucose showed that the diabetes model was successfully prepared.The control group was fed with conventional feed.After successful model establishment,the rats in the Moringa low and high dose groups were given 4.0 g/kg and 8.0 g/kg Moringa extract(Moringa flavone)by gavage everyday,the rats in the positive drug group were given piracetam 0.48 g/kg,and the rats in the model group and control group were given the same amount of normal saline once a day for 30 days.Morris water maze was used to evaluate the cognitive impairment of the rats.The hippocampus of the rats was harvested 12 hours after the last administration,and the advanced glycation end product receptor(RAGE)and nuclear factor-κB(NF-κB)were detected by immunohistochemistry.The contents of acetylcholinesterase(AChE),advanced glycation end product(AGE)and choline acetyl transferase(ChAT)were detected by enzyme linked immunosorbent assay(ELISA).Results Compared with the control group,the escape latency and the exploration distance in model group were extended,target quadrant stay time was shortened,the levels of AChE and AGE in brain tissue were significantly increased,and ChAT level was significantly decreased.Morris water maze experiment showed that compared with the model group,in the Moringa low and high dose groups from the 3rd day,the escape latency(s:35.07±7.21,33.14±5.35 vs.43.09�

关 键 词：辣木黄酮 糖尿病脑病 神经病理指标 认知功能 免疫组化 

分 类 号：R285.5[医药卫生—中药学]