酶法结合超声辅助提取桃儿七鬼臼毒素工艺研究  

Process of Enzyme Combined with Ultrasonic Assisted Extraction of Podophyllotoxin from Sinopodophyllum hexandrum

作　　者：许显莉 刘盈盈 冯海生[1,2] 马世震 李彩霞[1,2] Xu Xianli;Liu Yingying;Feng Haisheng;Ma Shizhen;Li Caixia(Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008,China;Qinghai Key Lab.of Qinghai-Tibet Plateau Biological Resources,Xining 810008,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院西北高原生物研究所,青海西宁810008 [2]青海省青藏高原特色生物资源研究重点实验室,青海西宁810008 [3]中国科学院大学,北京100049

出　　处：《中国野生植物资源》2021年第2期1-9,14,共10页Chinese Wild Plant Resources

基　　金：青海省应用基础研究(2019-ZJ-7061)。

摘　　要：目的:研究酶法结合超声辅助提取桃儿七鬼臼毒素工艺条件,并比较不同提取方法下鬼臼毒素提取得率。方法:采用单因素试验研究提取工艺中加酶量、酶解pH、酶解温度、超声时间、甲醇体积分数和料液比对鬼臼毒素提取得率的影响,并通过Plackett-Burman设计筛选出三个影响显著的因素;其次,采用响应面法对提取工艺参数进行进一步优化;最后,比较酶法结合超声辅助提取法和其他提取方法(加热回流提取、索式提取、超声提取、酶法提取)下鬼臼毒素提取得率。结果:酶法结合超声辅助提取鬼臼毒素的最佳工艺条件为:复合加酶量0.004 g,酶解pH为3,酶解温度30℃,料液比1∶30(g/mL)、甲醇体积分数为50%、超声时间10 min。在此条件下,鬼臼毒素提取得率为5.20%。结论:与其他提取方法相比,酶法结合超声辅助提取工艺耗时短,耗能低,且具有较好的提取效果。Objective:To study the ultrasonic assisted extraction method combined with the use of enzyme extract podophyllotoxin(PPT)from Sinopodophyllum hexandrum,and the extraction yield of PPT under different methods was compared.Methods:A single factor experiment was used to investigate the effects of the amount of added enzyme,pH of enzymatic hydrolysis,temperature of enzymatic hydrolysis,ultrasonic time,methanol volume fraction,and material-liquid ratio on the extraction yield of PPT in the extraction process,and Plackett-Burman design was used to select the significant affected factors.Secondly,the extraction process parameters were further optimized by response surface method.Finally,the PPT yield obtained by ultrasonic-assisted extraction combined with the use of enzyme was compared with other extraction methods(heating reflux extraction,Soxhlet extraction,ultrasonic extraction,and enzymatic extraction).Results:The optimized conditions:the amount of added enzyme was 0.004 g,the enzymatic hydrolysis pH was 3,the enzymatic hydrolysis temperature was 30℃,the material-liquid ratio was 1:30(g/mL),the methanol volume fraction was 50%,and the ultrasonic time was 10 min.Under these conditions,the maximum yield of extraction was 5.20%.Conclusion:Compared with other extraction methods,the ultrasonic-assisted extraction combined with the use of enzyme has a better extraction effect.

关 键 词：响应面法 酶法结合超声 提取 桃儿七 鬼臼毒素 

分 类 号：R932[医药卫生—生药学]