迷迭香酸血浆蛋白结合及冰片对结合率的影响研究  

Binding Rates of Rosmarinic Acid to Plasma Protein and the Impact of Borneol on the binding

作　　者：吴燕[1] 杨薇[1] 李彬[1] 雷玲[1] 胡竟一[1] 陈雏[1] 李东晓[1] Wu Yan;Yang Wei;Li Bin;Lei Ling;Hu Jingyi;Chen Chu;Li Dongxiao(Sichuan Academy of Chinese Medicine Science,Sichuan Key Laboratory of Quality and Innovation Chinese Materia Medica,Chengdu 610041)

机构地区：[1]四川省中医药科学院,中药材品质及创新中药研究四川省重点实验室,成都610041

出　　处：《中药药理与临床》2020年第6期83-86,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：四川省科技厅重点研发计划项目(编号:2018SZ0080、2019YFS0178)。

摘　　要：目的:研究迷迭香酸与血浆蛋白的结合率及冰片对结合的影响。方法:采用超高效液相色谱-二极管阵列检测器(UPLC-DAD)方法对未加或加入冰片后迷迭香酸与大鼠血浆的蛋白结合率进行测定。色谱柱Thermo Hypersil GOLD(50 mm×2.1 mm,3μm);流动相为乙腈-0.1%甲酸水溶液(25:75,v/v),流速0.5 mL/min;检测波长330 nm,柱温30℃。结果:迷迭香酸(25、10、4μg/mL)与大鼠血浆蛋白结合率分别为(97.19±0.06)%、(96.94±0.41)%、(96.86±0.06)%,加入25μg/mL冰片后迷迭香酸与大鼠血浆蛋白的结合率分别为(94.81±3.21)%、(95.23±2.58)%、(96.21±0.33)%。结论:不同浓度的迷迭香酸与大鼠血浆蛋白的结合率无明显差异,加入冰片对迷迭香酸的血浆蛋白结合率影响很小。Objective:To study the binding rate of rosmarinic acid to rat plasma protein and the impact of borneol on the binding.Methods:The plasma protein binding rates of rosmarinic acid with borneol or without borneol were determined by ultrafiltration combined with ultra-high performance liquid chromatography(UPLC-DAD).The chromatographic conditions were as follows:Thermo Hypersil GOLD column(50 mm×2.1 mm,3μm),acetonitrile-0.1%formic acid(25:75,v/v),flow rate 0.5 mL/min,wavelength 330 nm,column temperature 30℃.Results:The plasma protein binding rates of rosmarinic acid(25,10,4μg/mL)were(97.19±0.06)%,(96.94±0.41)%,(96.86±0.06)%,respectively.In the presence of borneol(25μg/mL),the binding rates of rosmarinic acid were(94.81±3.21)%,(95.23±2.58)%,(96.21±0.33)%,respectively.Conclusion:The binding rates of different concentrations of rosmarinic acid to rat plasma protein are similar,and borneol has no significant impact on the rat plasma protein binding rates of rosmarinic acid.

关 键 词：迷迭香酸 冰片 血浆蛋白结合率 超滤法 

分 类 号：R285.5[医药卫生—中药学]