丙戊酸钠对50%TBSAⅢ度烫伤大鼠心肌细胞凋亡的影响  

The effect of valproic acid sodium on apoptosis of myocardial cells in rats with 50%TBSAⅢ°scald injury

作　　者：孟祥熙 温海玲[1] 吴晓明[1] 胡森[2] MENG Xiangxi;WEN Hailing;WU Xiaoming;HU Sen(Department of Burn and Plastic Surgery,the Affiliated Hospital of Chengde Medical University,Chengde,Hebei 067000,China;Laboratory of Shock and Multiple Organ Disorders,Institute of Burns,Fourth Medical Center,PLA General Hospital,Beijing 100048,China)

机构地区：[1]承德医学院附属医院烧伤整形科,河北承德067000 [2]解放军总医院第四医学中心烧伤研究所休克与多器官障碍实验室,北京100048

出　　处：《重庆医学》2021年第5期732-736,共5页Chongqing medicine

基　　金：国家自然科学基金面上项目(81471872)。

摘　　要：目的研究丙戊酸钠(VPA)对致死性烫伤大鼠心肌细胞凋亡的影响。方法48只雄性SD大鼠分为3组,假烫组:采用37℃水浴,浸泡后腹腔内注射0.25 mL生理盐水,烫伤组:100℃水浴中背部浸泡15 s、腹部浸泡8 s,烫伤后腹腔内注射0.25 mL生理盐水;VPA组:烫伤后腹腔注射VPA治疗(300 mg/kg,溶于0.25 mL生理盐水)。分别于伤后3、6 h两个时间点取腹主动脉血,测定血浆肌酸激酶同工酶(CK-MB)水平;然后处死动物取心肌组织,TUNEL方法检测心肌细胞凋亡率,Western blot测定心肌组织内诱导型一氧化氮合酶(iNOS)表达,NO检测试剂盒测定心肌内NO水平、caspase-3活性试剂盒测定心肌内caspase-3活性。结果与假烫组比较,烫伤组伤后3 h和伤后6 h组CK-MB水平、心肌细胞凋亡率明显上升,caspase-3活性明显增加,iNOS表达水平、NO水平明显增高(均P<0.05);VPA组CK-MB水平、心肌细胞凋亡率与烫伤组比较明显下降,caspase-3活性明显降低,iNOS表达水平、NO水平明显减少,差异有统计学意义(均P<0.05)。结论在致死性烫伤大鼠治疗中,VPA能降低CK-MB水平,减少心肌细胞凋亡,保护心脏功能,可能与降低心肌组织内caspase-3活性、iNOS表达水平、NO水平有关。Objective To study the effect of valproic acid sodium(VPA)on apoptosis of myocardial cells in rats with fatal scald.Methods Forty-eight male Sprague-Dawley rats were randomly divided into 3 groups,the sham scald group(used 37℃water bath,intraperitoneal injection of 0.25 mL of normal saline),the scald group(100℃water bath soaked the back for 15 s,the abdomen soaked for 8 s,intraperitoneal injection of 0.25 mL normal of saline after scald),the VPA group[VPA treatment(300 mg/kg,dissolved in 0.25 mL of normal saline)after scald].Abdominal aortic blood was taken at 3 and 6 h after injury,and the plasma creatine kinase isoenzyme(CK-MB)level was measured;then the animals were sacrificed to take myocardial tissue,and TUNEL method was used to detect the apoptosis rate of myocardial cells,the expression of iNOS protein in the myocardial tissue was measured by Western blot,the NO level in the myocardium was measured by the NO detection kit,and the caspase-3 activity in the myocardium was measured by the caspase-3 activity kit.Results Compared with the sham scald group,the CK-MB level and myocardial cell apoptosis rate in the scald group were significantly increased at 3 h and 6 h after the injury,the activity of caspase-3 was significantly increased,and the expression level of iNOS protein and NO level were significantly increased,the differences were statistically significant(all P<0.05).While the CK-MB level and myocardial cell apoptosis rate in the VPA group were significantly lower than those in the scald group,caspase-3 activity was significantly reduced,iNOS protein expression level and NO level were significantly reduced when compared with the scald group,and the differences were statistically significant(all P<0.05).Conclusion In the treatment of fatal scalded rats,VPA can reduce the level of CK-MB and the apoptosis of myocardial cells,and protect the heart function,which may be related to the reduce of caspase-3 activity,iNOS protein expression level and NO level in myocardial tissue.

关 键 词：烫伤 丙戊酸 肌细胞 心脏 细胞凋亡 大鼠 SPRAGUE-DAWLEY 

分 类 号：R644[医药卫生—外科学]