抗结核药物超敏反应患者PBMCs中T淋巴细胞亚群及CD69、CD40L的表达意义  

The expression significance of T cell subsets,CD69 and CD40L in PBMCs of patients with hypersensitivity to antituberculosis drugs

作　　者：吴于青[1] 姜国强 刘㑇 周燕红[1] 张梁伟 WU Yuqing;JIANG Guoqiang;LIU Zhou;ZHOU Yanhong;ZHANG Liangwei(Department of Tuberculosis,Jiangxi Chest Hospital,Nanchang,Jiangxi 330006,China)

机构地区：[1]江西省胸科医院结核科,南昌330006

出　　处：《重庆医学》2021年第5期784-786,790,共4页Chongqing medicine

基　　金：江西省科技厅重点研发计划(20192BBGL70011);国家自然科学基金地区项目(81960003)。

摘　　要：目的研究抗结核药物超敏反应患者外周血单个核细胞(PBMCs)中T淋巴细胞亚群及表面分子CD69、CD40L的表达意义,探讨T淋巴细胞亚群及表面分子CD69、CD40L与抗结核药物超敏反应的关系。方法筛选该院初治肺结核治疗过程中出现超敏反应的患者60例为病例组,匹配同期抗结核治疗过程中未出现超敏反应的60例患者为对照组,采集外周静脉血并分离PBMCs,通过流式细胞仪检测PBMCs中Th1、Th2、CD4^(+)CD25^(+)Treg亚群及CD40L和CD69两种蛋白的表达。结果病例组患者PBMCS中的CD4+IFN-γ+Th1细胞比例较对照组明显升高(P<0.01),CD4^(+)IL-4^(+)Th2细胞比例较对照组升高,但差异无统计学意义(P>0.05)。与对照组比较,病例组患者外周血CD4^(+)CD25^(+)Treg比例降低(P<0.05),细胞表面CD40L和CD69的表达水平明显升高(P<0.05)。结论T淋巴细胞亚群比例失衡在抗结核药物超敏反应发生过程中可能起着重要作用。Objective To study the expression significance of T cell subsets and surface molecules CD69,CD40L in peripheral blood mononuclear cells(PBMCs)of patients with hypersensitivity to anti-tuberculosis drugs,and to explore the relationship between T cell subsets and surface molecules CD69,CD40L and hypersensitivity to antituberculosis drugs.Methods Sixty patients who had hypersensitivity reaction in the course of initial treatment of pulmonary tuberculosis in this hospital were selected as the case group,and 60 patients who did not have hypersensitivity reactions during the same period were selected as the control group.Peripheral venous blood was collected and PBMCs were separated.The expression of Th1,Th2,CD4^(+)CD^(+)25 Tregs and CD40L^(+)CD69^(+)were detected by flow cytometry.Results The expression of CD4+IFN-γ+Th1 cells in the case group was significantly higher than that in the control group(P<0.01),and the expression of CD4^(+)IL-4^(+)Th2 cells was lower than that in the control group,but the difference was not statistically significant(P>0.05).Compared with the control group,the proportion of CD4^(+)CD25^(+)T cells in the peripheral blood of the case group was decreased significantly(P<0.05),and the expressions of CD40L and CD69 on the cell surface were increased significantly(P<0.05).Conclusion The imbalance of T cell subsets may play an important role in the hypersensitivity of antituberculosis drugs.

关 键 词：结核 肺 超敏反应 T淋巴细胞亚群 免疫 细胞 

分 类 号：R969[医药卫生—药理学]