脂肪血浆制备冷沉淀的可行性研究  

Feasibility study of preparing cryoprecipitate from fatty plasma

作　　者：曹鸿霖 刘晋洪 曾凌晓 徐筠聘 李庚娣 郑瑶玲 CAO Honglin;LIU Jinhong;ZENG Lingxiao;XU Yunpin;LI Gengdi;ZHENG Yaolin(Operations Branch,Central Blood Station of Baoan District,Shenzhen,Guangdong 518101,China;Deputy Director′s Office,Blood Center of Shenzhen City,Shenzhen,Guangdong 518035,China;Department of Blood Supply Service,Central Blood Station of Baoan District,Shenzhen,Guangdong 518101,China;Shenzhen Blood Transfusion Medicine Institute,Shenzhen,Guangdong 518035,China;Department of Quality Control,Central Blood Station of Baoan District,Shenzhen,Guangdong 518101,China;Department of Blood Donation Service,Central Blood Station of Baoan District,Shenzhen,Guangdong 518101,China)

机构地区：[1]广东省深圳市宝安区中心血站业务科,518101 [2]广东省深圳市血液中心副主任室,518035 [3]广东省深圳市宝安区中心血站供血服务科,518101 [4]广东省深圳市血液输血医学中心研究所,518035 [5]广东省深圳市宝安区中心血站质管科,518101 [6]广东省深圳市宝安区中心血站献血服务科,518101

出　　处：《重庆医学》2021年第5期791-794,799,共5页Chongqing medicine

基　　金：广东省自然科学基金项目(2020A1515010387);深圳市科技创新委员会资助项目(JCYJ20160428164924181)。

摘　　要：目的研究脂肪血浆是否可以作为制备冷沉淀的原料浆,提高血浆的利用率,节约血液资源。方法选取轻、中、重度脂肪血浆作为原料浆制备冷沉淀,测定其纤维蛋白原(Fib)及Ⅷ因子(FⅧ)水平,分别计算各组冷沉淀中的Ⅷ因子和Fib合格率,经统计学分析,判断其质量能否达到国标要求,外观是否符合国标要求,同时检测原料浆及冷沉淀中三酰甘油(TG)水平,并进行统计学比较分析。结果轻、中、重度组脂肪血浆制备的冷沉淀FⅧ合格率分别为92%、6%、0,Fib合格率均为100%;轻度组脂肪血浆制备的冷沉淀FⅧ水平符合国标要求,中、重度组制备的冷沉淀FⅧ水平均不符合国标要求,Fib水平各组均达标;外观判定中、重度组制备的冷沉淀混有乳糜微粒不符合要求,轻度组符合要求;原料浆与制备后的冷沉淀中TG水平比较,轻、中度组差异有统计学意义(P<0.05),重度组差异无统计学意义(P>0.05)。结论轻度脂肪血浆尚可作为制备冷沉淀的原料浆,中、重度脂肪血浆则不能。Objective To study whether fat plasma can be used as raw material plasma for cryoprecipitate,to improve the utilization rate of plasma and save blood resources.Methods Mild,moderate and severe fat plasma were selected as raw material to prepare cryoprecipitate,and the levels of fibrinogen(Fib)and factorⅧ(FⅧ)were determined,and the qualification rates of factorⅧand Fib in each group of cryoprecipitate were calculated respectively.Whether its quality and appearance meet the requirements of the national standard were determined by statistical analysis.At the same time,the level of triacylglycerol(TG)in the raw material plasma and cryoprecipitate was detected,and statistical comparison and analysis were performed.Results The qualified rates of FⅧprepared from fat plasma in the mild,moderate,and severe groups were 92%,6%and 0,respectively,and the qualified rate of Fib was all 100%.The FⅧlevel in the cryoprecipitate prepared from fat plasma in the mild group met the requirements of the national standard,while the FⅧlevel in the moderate and severe groups did not meet the requirements of the national standard,and the Fib level in the cryoprecipitate in each group met the standard.Appearance judged that the cryoprecipitate prepared from the moderate and severe groups were mixed with chylomicrons,which did not meet the requirements,and the mild group met the requirements.The TG levels of the raw material plasma in the mild and moderate groups were compared with the cryoprecipitate,and the differences were statistically significant(P<0.05),the difference in severe group was not statistically significant(P>0.05).Conclusion Mildly fat plasma can still be used as the raw material slurry for cryoprecipitate,while medium and severely fatty plasma are not.

关 键 词：血样采集 脂肪血浆 离心法 血液报废 乳糜微粒 冷沉淀 

分 类 号：R457.14[医药卫生—治疗学]