活体监测植物葡萄糖的微型传感器研究  

Study on microsensor for monitoring of plant glucose in vivo

作　　者：杨磊 陈达 王成[1] 李爱学[1] YANG Lei;CHEN Da;WANG Cheng;LI Aixue(Beijing Research Center of Intelligent Equipment for Agriculture,Beijing 100097,China;College of Electronic and Information Engineering,Shandong University of Science and Technology,Qingdao 266590,China)

机构地区：[1]北京农业智能装备技术研究中心,北京100097 [2]山东科技大学电子信息工程学院,山东青岛266590

出　　处：《传感器与微系统》2021年第3期8-11,15,共5页Transducer and Microsystem Technologies

基　　金：北京市自然科学基金面上资助项目(2182022);国家自然科学基金面上资助项目(21974012)。

摘　　要：葡萄糖是植物体内的一种重要的可溶性糖,为了实时监测植物活体内葡萄糖的含量变化,开发了一种新型葡萄糖微型电化学生物传感器。利用葡萄糖氧化酶(GOD)催化葡萄糖,通过醋酸纤维素(CA)提高葡萄糖氧化酶的固载量,通过聚氨酯-环氧树脂(PU-Epoxy)提高传感器的选择性和稳定性。该传感器线性范围宽,检测限低,并且具有较高的灵敏度和选择性、良好的重现性及稳定性。并利用所研制的生物传感器成功地对芦荟体内的葡萄糖含量变化进行了实时检测。In this work,a new micro-electrochemical biosensor for glucose is developed for real-time monitoring of plant glucose in vivo.The glucose oxidase(GOD)is used to catalyze glucose.The cellulose acetate(CA)is used to increase the immobilization amount of GOD.The polyurethane-epoxy resin(PU-Epoxy)is used to improve the selectivity and stability of the sensor.The sensor has the advantages of wide linear range,low detection limit,high sensitivity and selectivity,and good reproducibility and stability.The as-prepared biosensor is successfully used to real-time monitoring the glucose in aloe in real-time and in vivo.

关 键 词：植物葡萄糖 生物传感器 活体 实时监测 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置]