17β-雌二醇通过ERK1/2途径诱导绵羊输卵管上皮细胞β-防御素-1的表达  

The Role of ERK1/2 Signaling Pathway on the β-Defensin-1 Gene Expression by 17β-Estrodiol in Sheep Oviduct Epithelial Cells

作　　者：王东阳 白萨日娜 李雅静 纳仁高娃 包图雅 WANG Dong-yang;BAI SA RI NA;LI Ya-jing;NA REN GAO WA;BAO TU YA(Shool of Basic Medical,Inner Mongolia Medical University,Huhhot 010110 China)

机构地区：[1]内蒙古医科大学基础医学院,内蒙古呼和浩特010110

出　　处：《内蒙古医学杂志》2021年第2期129-132,F0002,共5页Inner Mongolia Medical Journal

基　　金：内蒙古自然科学基金项目(编号:2019MS03008);内蒙古医科大学实验室开放基金项目(编号:2019ZN28);内蒙古医科大学精品在线开放课程课题(编号:NYJPZXKC2019021);内蒙古医科大学大学生创新创业训练计划项目(编号:201910132042);内蒙古自治区研究生科研创新项目(编号:S20191204Z);内蒙古医科大学2020年度校级教育教学改革项目(编号:NYJXGG2020147);2019年度自治区留学人员创新创业支持启动计划。

摘　　要：目的探讨17β-雌二醇(E_(2))对输卵管上皮细胞β-防御素-1(SBD-1)mRNA基因表达的影响以及相关信号通路作用机制。方法利用10^(-8) mol/L E_(2)培养绵羊输卵管上皮细胞6 h,分别添加MAPK/ERK1/2信号通路阻断剂和PI3K信号通路阻断剂,通过RT-qPCR技术检测SBD-1 mRNA的表达变化。结果 E_(2)可以上调绵羊输卵管上皮细胞中SBD-1 mRNA表达(P<0.05);添加ERK1/2信号通路阻断剂能够抑制了E_(2)诱导的SBD-1 mRNA的表达(P<0.05);添加PI3K信号通路阻断剂后SBD-1 mRNA表达量无显著差异(P>0.05)。结论 E_(2)诱导的SBD-1 mRNA的表达与ERK1/2信号通路有关,而PI3K信号通路可能不参与。Objective To investigate the effect of estrodiol(E_(2))on the expression of beta-defasin-1(SBD-1)mRNA in oviduct epithelial cells and the mechanism of related signaling pathways.Methods Sheep oviduct epithelial cells were cultured with 10-8 mol/L E_(2) for 6 h in ourexperiment,then added MAPK/ERK1/2 signaling pathway blocker and PI3 K signaling pathway blocker respectively,in order to screen the expression changes of SBD-1 mRNA we use the RT-qPCR.Results SBD-1 mRNA expression in sheep oviduct epithelial cells induced by E2 was increased(P<0.05).Addition of MAPK signaling pathway blocker significantly inhibited E2-induced SBD-1 mRNA expression(P<0.05),while there was no significant difference in the expression level of SBD-1 mRNA after PI3 K signaling pathway blocker was added(P>0.05).Conclusion The expression of SBD-1 mRNA in E2 induced sheep oviduct epithelial cells is mediated by ERK1/2 signaling pathway and PI3 K signaling pathway may not be involved.

关 键 词：输卵管上皮细胞 β-防御素-1 17Β-雌二醇 信号通路 

分 类 号：R339.22[医药卫生—人体生理学]