三氧化二砷对鸡肝脏氧化应激和蛋氨酸亚砜还原酶表达的影响  

Effects of Arsenic Trioxide Treatment on Oxidative Stress and Methionine Sulfoxide Reductase Expression in Chicken Liver

作　　者：伍少峰 钟高龙 万方 宁芷君 蒋璇璇 胡婷 陈忠伟 赵武 卢冰霞 唐兆新[1] 何颖 胡莲美[1] WU Shaofeng;ZHONG Gaolong;WAN Fang;NING Zhijun;JIANG Xuanxuan;HU Ting;CHEN Zhongwei;ZHAO Wu;LU Bingxia;TANG Zhaoxin;HE Ying;HU Lianmei(College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China)

机构地区：[1]华南农业大学兽医学院,广州510642 [2]广西壮族自治区兽医研究所广西兽医生物技术重点实验室,南宁530001

出　　处：《畜牧兽医学报》2020年第11期2867-2874,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31402264);广西创新驱动发展专项(桂科AA17204057-1,桂科AA18118051);柳州市科学研究与技术开发计划项目(2018BH20501);广西南宁市西乡塘区科学研究与技术开发计划项目(201810204);玉林市科学研究与技术开发计划项目(玉市科201934022)。

摘　　要：本研究旨在探讨三氧化二砷(ATO)对鸡肝脏氧化应激和蛋氨酸亚砜还原酶(Msrs)表达的影响。将32只1日龄雏鸡分为对照组(生理盐水)、低剂量组(1 mg·kg^(-1) ATO溶液)、中剂量组(3 mg·kg^(-1) ATO溶液)和高剂量组(9 mg·kg^(-1)ATO溶液),用相应浓度的ATO溶液通过灌胃处理雏鸡,每天灌胃1次,持续5周。试验期结束后,取肝组织并分析肝脏系数和观察肝组织病理变化;测定肝组织中MDA水平和SOD活力;检测Msrs基因和蛋白的表达水平。结果显示,与对照组相比,中、高剂量组肝脏系数极显著升高(P<0.01),中、高剂量组肝有淤血和水泡变性;相比于对照组,试验组MDA水平极显著增加(P<0.01),SOD活力极显著下降(P<0.01);低、中、高剂量组中MsrA、MsrB1、MsrB3基因mRNA表达水平相比对照组极显著增加(P<0.01或P<0.001),但MsrA蛋白水平极显著降低(P<0.01)。结果表明,ATO诱导鸡肝发生氧化应激,同时通过促进Msrs表达以清除ROS,减轻肝氧化损伤。This study aimed to investigate the effect of arsenic trioxide(ATO)on oxidative stress and methionine sulfoxide reductase(Msrs)expression in chicken liver.A total of 32 one-day-old chicks were divided into control group(saline solution),low-dose group(1 mg·kg^(-1) ATO solution),medium-dose group(3 mg·kg^(-1) ATO solution)and high-dose group(9 mg·kg^(-1) ATO solution).Chickens were treated by gavage with ATO solution of corresponding concentration once a day for 5 weeks.The liver coefficient,histopathological sections,MDA level,SOD activity,Msrs gene and protein expression levels of liver were analyzed.The results showed that compared with the control group,the liver coefficient of the medium-and high-dose groups were significantly increased(P<0.01),and the liver congestion and vesicular degeneration were found in the medium-and high-dose groups.Meanwhile,the experimental groups had significantly increased MDA levels(P<0.01)and decreased SOD activity(P<0.01)as compared with the control group;MsrA,MsrB1,MsrB3 gene mRNA expression levels in the ATO treatment groups were significantly increased compared with the control group(P<0.01 or P<0.001);However,the protein levels of MsrA were reduced significantly(P<0.01).The results indicated that ATO induced oxidative stress in the liver of chickens,and at the same time promoted the expression of Msrs in liver to clear ROS and reduce liver oxidative damage.

关 键 词：三氧化二砷 Msrs 鸡 肝 氧化应激 

分 类 号：S856.9[农业科学—临床兽医学]