肾复康颗粒对人工诱发鸡肾肿病理模型的炎性细胞因子及IL-1R/NF-κB信号通路的影响  

The Effect of Shenfukang Granule on Content of Inflammatory Cytokines and the Signaling Pathway of IL-1R/NF-κB Induced by the Artificial Pathological Model of Chicken Kidney Swelling

作　　者：罗艺晨[1,2,3] 杨庆昌 林春发 叶芮伶 尚杨斐 胡宇 刘娟[1,2,3] LUO Yichen;YANG Qingchang;LIN Chunfa;YE Ruiling;SHANG Yangfei;HU Yu;LIU Juan(College of Animal Science, Southwest University, Chongqing 402460, China;Chinese Veterinary Herbal Drugs Innovation Research Laboratory, University Veterinary Science Engineering Research Center in Chongqing, Chongqing 402460, China;Center for Immune Research, School of Medicine, Southwest University, Chongqing 402460, China)

机构地区：[1]西南大学动物科学学院,重庆402460 [2]重庆市高校兽医科学工程研究中心中兽药创新研发实验室,重庆402460 [3]西南大学医学院免疫研究中心,重庆402460

出　　处：《畜牧兽医学报》2020年第11期2886-2894,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技基础专项(2013FY110600-03)。

摘　　要：旨在探讨肾复康颗粒对鸡肾肿的抗炎机制。将90只健康雏鸡分为空白组(15只)和试验组(75只),试验组通过饲喂自制高钙高蛋白饲料人工复制鸡肾肿模型,造模成功后,随机分为模型组、阳性药物组、肾复康颗粒高、中、低剂量组,每组15只,肾复康颗粒高、中、低剂量组分别饮水给药肾复康颗粒2.0、1.0、0.5 g·L^(-1),阳性药物组饮水给药苍蓝口服液1.0 mL·L^(-1),模型组饮水给予生理盐水1.0 mL·L^(-1),2次·d-1,连用5 d。分别于造模前、后以及末次给药后2 d进行翅下静脉采血,分离血清,采用ELISA法检测血清IL^(-1)、TNF-α、MCP-1、TGF-β1的含量变化;采用RT-PCR法检测肾IL^(-1)R、IκBα、NF-κBp65 mRNA的转录。结果显示:与空白组相比,模型组雏鸡血清IL^(-1)、TNF-α、MCP-1、TGF-β1含量显著升高(P<0.05),肾中IL^(-1)R、NF-κBp65 mRNA转录量极显著升高(P<0.01),肾中IκBαmRNA转录量极显著降低(P<0.01)。饮水给药治疗后,肾复康颗粒高、低剂量组和阳性药物组雏鸡血清IL^(-1)、TNF-α、MCP-1、TGF-β1含量显著降低(P<0.05);肾复康颗粒中剂量组肾中IL^(-1)R、NF-κBp65 mRNA转录量极显著降低(P<0.01),肾中IκBαmRNA转录量极显著增加(P<0.01)。肾复康颗粒通过降低肾肿鸡血清中IL^(-1)、TNF-α、MCP-1、TGF-β1中含量,抑制IL^(-1)R/NF-κB信号通路,从而发挥抗炎作用。The objective of the current study was to explore the anti-inflammatory mechanism of Shenfukang granule on chicken kidney swelling.Ninety healthy chickens were divided into blank group(n=15)and test group(n=75),the test group was replicated by the chicken kidney swelling model through feeding high-calcium and high-protein diet.After successful modeling,the test group was randomly divided into model group,positive drug group,Shenfukang high,medium and low dose group(n=15),Shenfukang granule 2.0,1.0 and 0.5 g·L^(-1),positive drug group,drinking water to Canglan oral liquid 1.0 mL·L^(-1),The drinking water of the model group was given 1.0 mL·L^(-1) of normal saline for 2 times·d-1 and 5 days.Subpteral venous blood was collected before modeling,after modeling and 2 days after the last administration to isolate the serum.The content changes of serum IL^(-1),TNF-α,MCP-1 and TGF-β1 content were detected by ELISA.RT-PCR was used to detect renal IL^(-1)R,IκBα,NF-κB mRNA transcription.Results were as follows:Compared with the blank group,the serum contents of IL^(-1),TNF-1,MCP-1 and TGF-β1 were significantly increased in the model group(P<0.05),the transcription of IL^(-1)R,NF-κBp65 mRNA in kidney was significantly increased(P<0.01),and IκBαmRNA in kidney was significantly decreased(P<0.01).After drinking water treatment,the serum IL^(-1),TNF-α,MCP-1 and TGF-β1 contents of Shenfukang granule were significantly decreased in the high and low dose group and the positive drug group(P<0.01).The results of the current study suggested that Shenfukang granule played an anti-inflammatory role in inhibiting the IL^(-1)R/NF-κB signaling pathway by reducing the contents of IL^(-1),TNF-α,MCP-1 and TGF-β1 in serum of kidney swelling chicken.

关 键 词：肾复康颗粒 肾肿 抗炎 转录 IL-1R/NF-κB信号通路 

分 类 号：S853.9[农业科学—临床兽医学]