人中性粒细胞载脂蛋白和中性粒细胞CD64在慢性前列腺炎患者前列腺液中的表达及其意义  

Expression and significance of human neutrophil lipocalin and neutrophil CD64 in prostatic fluid of chronic prostatitis patients with different degrees of infection

作　　者：韦毅 农君仁[1] 吴秋龙 卢国平 Wei Yi;Nong Jun-Ren;Wu Qiu-Long;Lu Guo-Ping(Department of Urology,Affiliated Ethnic Hospital of Guangxi Medical University,Nanning 530001,China;Department of Clinical Laboratory,Affiliated Ethnic Hospital of Guangxi Medical University,Nanning 530001,China)

机构地区：[1]广西医科大学附属民族医院泌尿外科,南宁530001 [2]广西医科大学附属民族医院检验科,南宁530001

出　　处：《解放军医学杂志》2021年第2期163-168,共6页Medical Journal of Chinese People's Liberation Army

摘　　要：目的探讨人中性粒细胞载脂蛋白(HNL)和中性粒细胞CD64(nCD64)在慢性前列腺炎患者前列腺液中的表达及其意义。方法选取2017年2月-2019年10月广西医科大学附属民族医院收治的慢性前列腺炎患者178例,根据是否感染细菌分为慢性前列腺炎/慢性盆腔疼痛综合征组(CP/CPPS组,n=40)与慢性细菌性前列腺炎组(CBP组,n=138),然后根据美国国立卫生研究院慢性前列腺炎症状指数(NIH-CPSI)评分将CBP组分为轻度组(n=65)、中度组(n=46)与重度组(n=27);另选择同期在该院进行健康体检者40名作为对照组。记录并分析研究对象的年龄、体重指数(BMI)、病程、白细胞计数(WBC)及NIH-CPSI评分;采用ELISA法检测前列腺液中HNL的表达水平;流式细胞术检测前列腺液中nCD64的表达水平;Pearson相关法分析CBP组HNL、nCD64水平与NIH-CPSI评分的相关性;logistic回归法分析CBP、CP/CPPS的影响因素;ROC曲线评价HNL、nCD64对CBP的诊断价值。结果与对照组相比,CP/CPPS组、CBP组及其各亚组WBC、HNL、nCD64水平均明显升高(P<0.05),CBP组高于CP/CPPS组,且CBP各亚组随着感染程度的加重而升高,组间差异有统计学意义(P<0.05)。CP/CPPS组与CBP组NIH-CPSI评分差异无统计学意义(P>0.05);但中度组NIH-CPSI评分高于轻度组,重度组高于中度组和轻度组,差异有统计学意义(P<0.05)。Pearson相关分析结果显示,CBP组前列腺液中HNL、nCD64水平均与NIH-CPSI评分呈明显正相关(r=0.897,P<0.001;r=0.919,P<0.001)。多因素logistic回归分析显示,细菌感染、HNL及nCD64是CBP的独立危险因素(P<0.05),其他病原体感染、排尿功能失调、神经内分泌异常、免疫反应异常及盆腔相关疾病是CP/CPPS的独立危险因素(P<0.05)。ROC曲线分析结果显示,前列腺液中的HNL、nCD64诊断CBP的曲线下面积(AUC)分别为0.837和0.899,最佳临界值分别为165.36μg/L和4078.28个/细胞,两者联合诊断的AUC为0.949,具有更高的诊断价值。结论慢�Objective To investigate the expression and significance of human neutrophil lipocalin(HNL)and neutrophil CD64(nCD64)in prostatic fluid of chronic prostatitis patients with different infection degrees.Methods One hundred and seventy-eight patients with chronic prostatitis admitted to the Affiliated Ethnic Hospital of Guangxi Medical University from February 2017 to October 2019 were selected as the research objects.According to whether they were infected with bacteria,the patients were divided into chronic prostatitis/chronic pelvic pain syndromes group(CP/CPPS group,n=40)and chronic bacterial prostatitis group(CBP group,n=138).According to National Institutes of Health Chronic Prostatitis Symptom Index(NIH-CPSI)score,CBP group was divided into mild group(n=65),moderate group(n=46)and severe group(n=27),and another 40 healthy people were selected as control group.Age,body mass index(BMI),course of disease,white blood cell(WBC),and NIH-CPSI score were recorded and analyzed.The expression level of HNL in prostatic fluid was detected by enzyme linked immunosorbent assay(ELISA).The expression level of nCD64 in prostatic fluid was detected by flow cytometry.Pearson correlation was used to analyze the correlation between both HNL and NCD64 levels and NIH-CPSI score in the CBP group.Logistic regression analysis was used to analyze the influencing factors of CBP and CP/CPPS.ROC curve was used to evaluate the diagnostic value of HNL and nCD64 for CBP.Results Compared with the control group,WBC,HNL and nCD64 levels in CP/CPPS group,CBP group and its subgroups were significantly increased(P<0.05),while the CBP group was higher than CP/CPPS group,and the CBP subgroup increased with the increase of infection degree,and the difference between the groups was statistically significant(P<0.05).There was no significant difference in NIH-CPSI score between CP/CPPS group and CBP group(P>0.05),but NIH-CPSI score of moderate group was higher than that of mild group,and that of severe group was higher than that of moderate group and mi

关 键 词：人中性粒细胞载脂蛋白 中性粒细胞CD64 慢性前列腺炎 前列腺液 

分 类 号：R378[医药卫生—病原生物学]