甜瓜细菌性果斑病病菌鉴定及不同材料抗病性测定  

Identification of Bacterial Fruit Blotch Pathogen and Detection of Different Resistant Plant Materials in Melon

作　　者：刘梦华 卢霞 李腾 李永博 樊文义 司龙亭 李文虎 阿门 LIU Meng-hua;LU Xia;LI Teng(Jiangsu Greenport Modern Agriculture Development Co.,Ltd.,Suqian,Jiangsu 223800)

机构地区：[1]江苏绿港现代农业发展有限公司,江苏宿迁223800

出　　处：《安徽农业科学》2021年第6期145-148,共4页Journal of Anhui Agricultural Sciences

基　　金：2019年宿迁市产业发展引导资金(自然科学资金)(K201910)。

摘　　要：为明确近几年江苏地区甜瓜叶片及瓜上发生的某一细菌性病害,以江苏绿港现代农业发展有限公司设施大棚中甜瓜发病叶片为研究对象,经分离纯化得到菌株LG08,观察病菌形态初步判定该病原菌为甜瓜细菌性果斑病菌,为进一步明确该病菌,分别用4对细菌性果斑病菌通用标记(BX、BX-S、ERC和WFB)、3对甜瓜细菌性果斑病菌特异性标记(AcM380、AcM797、AcM13)及16S测序进行分析。结果表明,菌株LG08为燕麦嗜酸菌西瓜亚种(Acidovorax avenae subsp.citrulli)。苗期和坐果期对西甜瓜部分材料接种菌株LG08,结果发现菌株LG08对西甜瓜都有致病性。该研究不仅有利于进一步了解甜瓜细菌性果斑菌的特性,为果斑病的防治提供参考,而且也有助于甜瓜抗病品种的选育。To identify the pathogen causing leaves and fruits disease in melon these years in Jiangsu Province,diseased materials were sampled from solar greenhouse of Jiangsu Greenport Modern Agriculture Development Co.,Ltd..In this study,the isolated pathogen named LG08 was purified and initially identified as melon Bacterial Fruit Blotch(BFB)pathogen based on its colonies morphological characterization.Further,four pairs of commonly used BFB markers BX,BX-S,ERC and WFB as well as three pairs of melon specific BFB markers AcM380,AcM797 and AcM13 were used for molecular characterization.In addition,DNA sequencing of LG08 was performed based on 16S amplified fragments.Together,LG08 was ultimately confirmed as a strain of Acidovorax avenae Subsp.citrulli(Aac).It was found that LG08 was pathogenic to melon and watermelon upon inoculating it to some of their plants materials during seedling and fruit setting period.This study not only helps to more understanding the characteristics of BFB,but also provides resistant lines to melon breeding program while constituting a reference for the prevention and treatment of BFB.

关 键 词：甜瓜 细菌性果斑病菌 分离纯化 抗病材料 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]