黄瓜DnaJ基因家族鉴定及对高温胁迫的表达响应  

Identification of DnaJ gene family in cucumber and its expression response to high temperature stress

作　　者：宋蒙飞 王星 张开京[1] 陈劲枫[1] 娄群峰[1] SONG Mengfei;WANG Xing;ZHANG Kaijing;CHEN Jinfeng;LOU Qunfeng(State Key Laboratory of Crop Genetics and Germplasm Enhancement/College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室/园艺学院,江苏南京210095

出　　处：《南京农业大学学报》2021年第2期267-277,共11页Journal of Nanjing Agricultural University

基　　金：江苏省农业科技自主创新基金[CX(20)2019];江苏省农业重大新品种创制项目(PZCZ201719);江苏高校“青蓝工程”人才项目

摘　　要：[目的]本文旨在研究黄瓜热激蛋白DnaJ(CsDnaJ)的家族基因鉴定,并分析其在高温胁迫环境下的响应模式。[方法]利用生物信息学软件及黄瓜的全基因组测序数据库、公共数据库对CsDnaJ进行基因家族成员、染色体位置、蛋白特性、结构特征、进化及复制模式和组织表达特性分析,同时通过实时定量PCR技术分析其在高温胁迫环境下的响应模式。[结果]黄瓜CsDnaJ基因家族共存在81个成员基因,其中Chr 3含DnaJ基因最多,为16个,Chr 4、Chr 7最少,均为9个;CsDnaJ基因所编码的蛋白序列长度为113~2551 aa,且至少含有1个完整的J结构域核心区序列;亚细胞位置预测显示大部分蛋白定位于细胞核及叶绿体中。基因结构显示CsDnaJ基因均含有1~22个外显子。通过进化分析发现CsDnaJ蛋白可明显区分为9个亚簇,每个亚簇均存在拟南芥同源蛋白。共线性分析发现,CsDnaJ基因家族共存在17个共线基因,组成10对复制基因对,其中存在5对片段重复事件,3对串联重复事件,2对随机重复事件。组织表达特异性结果显示CsDnaJ基因在叶、茎、子房中的表达量略高于其在雄花、雌花、根及卷须中的表达量。荧光定量PCR结果显示,高温环境(昼/夜温度为42℃/35℃)可以诱导CsDnaJ基因的高表达,从而参与植物响应高温环境胁迫进程。[结论]黄瓜CsDnaJ基因家族核心结构域高度保守,在黄瓜响应高温胁迫中发挥重要作用。[Objectives]The paper aimed to identify the DnaJ protein family genes in cucumber and analyze its expression levels under high temperature stress.[Methods]Gene family members,chromosome position,protein characteristics,structural characteristics,evolution and replication patterns,tissue expression characteristics were identified using bioinformatics software,the cucumber reference genome database,and public databases,while their expression patterns under high temperature stress environment were analyzed by quantitative real-time PCR.[Results]A total of 81 CsDnaJ member genes of cucumber were identified.Chr 3 contained the largest number of DnaJ genes(16),and Chr 4 and Chr 7 contained the minimum(9).The length of the proteins sequence encoded by the CsDnaJ genes varied from 113 to 2551 aa,which contained at least 1 complete core region sequence of the J-domain.The prediction of subcellular location showed that most of the proteins were located in the nucleus or chloroplast.The structure of CsDnaJ genes showed that each gene contained 1 to 22 exons.Evolutionary analysis showed that CsDnaJ proteins could be distinguished into 9 subclusters,each of which contained homologous proteins in Arabidopsis thaliana.Collinearity analysis showed that there were 17 collinear genes in the CsDnaJ gene family,forming 10 pairs of duplicating gene pairs,including 5 pairs of fragment repeating events,3 pairs of tandem repeating events,and 2 pairs of random repeating events.The expression levels of CsDnaJ genes in leaf,stem and ovary were slightly higher than those in male flower,female flower,root and tendrils.Quantitative real-time PCR analysis showed that the high temperature environment(the temperature of day/night was 42℃/35℃)could induce the high expression of CsDnaJ genes to respond to high temperature stress environmental.[Conclusions]The core structure of the CsDnaJ gene family in cucumber is highly conserved and it plays an important role in response to high temperature stress in cucumber.

关 键 词：黄瓜 DnaJ基因 生物信息学 高温胁迫 表达分析 

分 类 号：S642.2[农业科学—蔬菜学]