利用重组自交系定位水稻种子低氧发芽力QTL  

Identification of QTL Involved in Anaerobic Germination Tolerance by RIL Population in Rice

作　　者：张所兵[1] 张云辉[1] 陈海元 林静[1] 汪迎节[1] 朱晓妹 宋春凤[2] 方先文[1] ZHANG Suobing;ZHANG Yunhui;CHEN Haiyuan;LIN Jing;WANG Yingjie;ZHU Xiaomei;SONG Chunfeng;FANG Xianwen(Institute of Crop Germplasm and Biotechnology,Jiangsu Academy of Agricultural Sciences,The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm,Nanjing 210014,China;Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing Botanical Garden,Sun Yat-sen Nanjing,Nanjing 210014,China)

机构地区：[1]江苏省农业科学院种质资源与生物技术研究所,江苏省农业种质资源保护与利用平台,江苏南京210014 [2]江苏省中国科学院植物研究所(南京中山植物园),江苏南京210014

出　　处：《华北农学报》2020年第6期31-35,共5页Acta Agriculturae Boreali-Sinica

基　　金：江苏省农业科技自主创新资金(CX(18)1001);2018年中医药公共卫生服务补助专项(财社[2018]43号)。

摘　　要：鉴定低氧发芽力强的水稻资源,进而挖掘控制低氧发芽的QTL,可以为改良直播稻低氧发芽能力提供材料基础和基因资源。在装满无菌水的10 cm高的离心管中鉴定了云南省籼稻地方品种扎西玛、江苏省优良食味粳稻品种南粳46及包含135个家系的扎西玛/南粳46 RIL群体的低氧发芽力。以28℃黑暗培养7 d的胚芽鞘长度作为低氧发芽力的评价标准。结果表明:在淹水7 d后,扎西玛和南粳46胚芽鞘长度分别为2.92,6.68 cm,二者在P=0.01水平上存在极显著差异。进而对扎西玛/南粳46 RIL群体135个家系低氧发芽力QTL分析发现,在水稻第12号染色体上的RM1300和RM1227之间检测到一个控制低氧发芽力的QTL(qAG-12)。该QTL的LOD值为3.04,可以解释11.24%的表型变异。生物信息学分析表明,qAG-12位于第12染色体上26.00~27.34 Mb的1.34 Mb范围内。该区域共有200多个注释基因,未见已报道控制低氧发芽力的QTL。以上结果表明:扎西玛和南粳46低氧发芽力存在极显著差异;qAG-12是一个新的控制低氧发芽力的QTL。Identifying rice resources with strong germination ability under anaerobic condition,and then mapping QTL related to anaerobic germination ability could provide materials and QTLs for improving the anaerobic germination ability of direct-seeded rice.The anaerobic germination ability of the indica local variety Zhaxima from Yunnan Province,the japonica variety Nanjing 46 with excellent taste from Jiangsu Province and the Zhaxima/Nanjing 46 RIL populations containing 135 lines were evaluated in centrifuge tubes(10 cm),which were filled with distilled water.The seeds were germinated at 28℃for 7 days in a dark germination chamber,and then the length of the coleoptile of the seeds was measured and used as the evaluation standard for the anaerobic germination ability.The results showed that after 7 days of flooding,the lengths of the coleoptiles of Zhaxima and Nanjing 46 were 2.92,6.68 cm,respectively,and there was extremely significant statistical difference between the two varieties at the P=0.01 level.Furthermore,the QTL related to anaerobic germination was mapped by RIL population consisting of 135 lines derived from a cross between Zhaxima and the Nanjing 46,which revealed that one QTL(qAG-12)correlated with anaerobic germination located on chromosome 12 between SSR marker RM1300 and RM1227.The qAG-12 with a LOD score of 3.04 explained about 11.24%of the phenotypic variation.Bioinformatics analysis showed that the qAG-12 was located in the range of 1.34 Mb between 26.00,27.34 Mb on chromosome 12.There were more than 200 predicted ORFs in this candidate region of qAG-12,and no reported QTL involved in anaerobic germination at the germination stage in the region.The above results indicated:the anaerobic germination ability exhibited extremely significant difference between Zhaxima and the Nanjing 46 at the level of P=0.01;The qAG-12 was a novel QTL correlated with anaerobic germination.

关 键 词：直播稻 重组自交系 低氧发芽 QTL定位 

分 类 号：S511.03[农业科学—作物学]