基于转录组学的安格斯牛和中国西门塔尔牛脂肪沉积相关差异表达基因研究  

Study on Differentially Expressed Genes Related to Fat Deposition Gene in Angus and Chinese Simmental Cattle Base on Transcriptome

作　　者：韩信嵘 吴慧光[1,2] 吴江鸿 王国富 高树新[1,3] HAN Xinrong;WU Huiguang;WU Jianghong;WANG Guofu;GAO Shuxin(College of Animal Science and Technology,Inner Mongolia University for Nationalities,Tongliao 028042,China;College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China;Institute of Cattle Genetics and Breeding,Inner Mongolia University for Nationalities,Tongliao 028000,China)

机构地区：[1]内蒙古民族大学动物科技学院,内蒙古通辽028042 [2]扬州大学兽医学院,江苏扬州225009 [3]内蒙古民族大学黄牛遗传繁育研究所,内蒙古通辽028000

出　　处：《华北农学报》2020年第6期208-216,共9页Acta Agriculturae Boreali-Sinica

基　　金：内蒙古“草原英才”工程专项;内蒙古自然科学基金面上项目(2019MS03077);内蒙古自治区高等学校科学技术研究项目(NJZC16189);国家自然科学基金项目(31660648)。

摘　　要：为探明安格斯牛和中国西门塔尔牛脂肪沉积的相关差异表达基因,以其背膘组织为研究对象,提取总RNA,建立测序文库后利用华大BGISEQ-500进行测序,经生物信息学分析筛选出差异表达基因,并对差异表达基因进行GO功能富集和KEGG通路分析,最后采用荧光定量qPCR(qRT-PCR)验证转录组测序数据的准确性。中国西门塔尔牛对安格斯牛转录组数据结果显示有1296个差异表达基因,其中上调基因802个,下调基因494个;对转录组数据的GO功能富集分析结果显示在生物过程、分子功能、细胞组分分别富集到2205,890,3149个条目,KEGG通路分析结果表明,差异基因显著富集在6个分支上。对其中与脂肪沉积相关的cAMP通路进行分析,筛选出与脂肪沉积相关的差异表达基因3个,分别为DKKL1、ACACB和PTGS2,并通过qRT-PCR对基因DKKL1、ACACB和PTGS2进行了验证,初步确定其为脂肪沉积的候选基因。通过对中国西门塔尔牛和安格斯牛的背膘组织测序获得的大量差异表达基因,为后续对脂肪沉积相关基因的筛选奠定了基础。To identify differentially expressed genes related to fat deposition in Angus and Chinese simmental cattle and analyze differentially expressed gene functions and pathways,the total RNA was extracted from the backfat,then sequenced by BGISEQ-500.The differentially expressed genes were screened by bioinformatics analysis,GO and KEGG enrichment analysis were used to study the function of differentially expressed genes and the candidate genes related to fat deposition were screened.The accuracy of RNA-seq results was verified by qRT-PCR.The results showed that there were 1296 significant differentially expressed genes in Angus cattle compared with Chinese simental cattle,including 802 up genes and 494 down genes.Differential transcripts of GO function enrichment analysis revealed that molecular functions,cell components,and biological processes were enriched to 890 entries,3149 entries,and 2205 entries.KEGG analysis showed that the differential transcripts were significantly enriched in 6 pathways.The cAMP pathway associated with fat deposition was analyzed,and the differentially expressed genes associated with fat deposition were screened out,the genes DKKL1,ACACB and PTGS2 were verified by qRT-PCR,and they were preliminarily identified as candidate genes for fat deposition.At the same time,a large number of differentially expressed genes were obtained by sequencing the backfat tissues of Chnese simmental cattle and Angus cattle,which laid a foundation for the subsequent screening of genes related to fat deposition.

关 键 词：牛 脂肪沉积 DKKL1 ACACB PTGS2 QRT-PCR 

分 类 号：Q78[生物学—分子生物学] S832[农业科学—畜牧学]