基于纳米金/多孔硅基底的荧光标记法对生物分子的检测  

Detection of Biological DNA by Rhodamine Fluorescent Labeling Based on Gold Nanoparticles/Porous Silicon Substrate

作　　者：何蕾[1] HE Lei(School of Physics Science and Technology,Xinjiang University,Urumqi Xinjiang 830046,China)

机构地区：[1]新疆大学物理科学与技术学院,新疆乌鲁木齐880046

出　　处：《新疆大学学报（自然科学版）（中英文）》2021年第2期159-162,167,共5页Journal of Xinjiang University(Natural Science Edition in Chinese and English)

基　　金：国家自然科学基金(61575168)。

摘　　要：为制做一种低成本、易标记、稳定性好、灵敏度高的生物传感器,本文制备了一种具有较强荧光信号及生物兼容性的多孔硅生物传感器材料.利用纳米金的等离子体效应增强多孔硅中荧光标记物的荧光信号,在纳米金修饰的多孔硅中采用罗丹明6G荧光标记法对生物分子进行检测.通过链接生物荧光探针后荧光信号强度的变化实现生物浓度检测,其检测极限为121 nM,对生物大分子成功实现了低成本、易标记、稳定性好、灵敏度高的检测.The purpose of this study is to prepare a low-cost,easy labeling,good stability and high sensitivity biosensor,a porous silicon biosensor material with strong fluorescence signal and biocompatibility was successfully prepared,the plasma effect of gold nanoparticles is used to enhance the fluorescence signal of fluorescent markers in porous silicon,Rhodamine 6 G fluorescence labeling method was used to detect biomolecules in modified porous silicon by gold nanoparticles.Through the change of fluorescence signal intensity after linking the biological fluorescence probe,the biological concentration can be detected,the detection limit is 121 nM,it can detect biomacromolecules with low cost,easy labeling,good stability and high sensitivity.

关 键 词：多孔硅 折射率 荧光 生物传感器 

分 类 号：Q63[生物学—生物物理学] O439[机械工程—光学工程]