集胞藻Synechocystis sp. PCC 6803脂质组的分析  

ANALYSIS OF LIPIDOMES OF THE SYNECHOCYSTIS SP.PCC 6803 CELLS

作　　者：郭晓烨 李艳华 韩丹翔[1,2] GUO Xiao-Ye;LI Yan-Hua;HAN Dan-Xiang(Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院水生生物研究所,武汉430072 [2]中国科学院大学,北京100049

出　　处：《水生生物学报》2021年第2期376-386,共11页Acta Hydrobiologica Sinica

摘　　要：以集胞藻Synechocystis sp.PCC 6803为研究对象,研究建立了基于超高效液相色谱耦合串联质谱技术脂质组学分析方法。鸟枪法脂质组学通过电喷雾离子化有效分离油脂粗提物中所含单个脂质分子,在三重四极杆扫描碎片离子,能够利用特征片段离子鉴定光合甘油酯的种类和酰基组成,具有高效、灵敏度高和质量准确度高等优点。对不同光强下生长的Synechocystis sp.PCC 6803细胞的各脂质组分进行了全定量分析,发现单半乳糖甘油二酯(Monogalactosyldiacylglycerol,MGDG)和磷脂酰甘油(Phosphatidyl glycerol,PG)在高光处理的第2小时即显著积累,增长量分别为34.64%和68.49%,其中以含有从头合成、高度饱和的脂肪酸的种类增长最为快速和显著,而后高不饱和度的脂肪酸组成的种类逐渐积累。双半乳糖甘油二脂(Digalactosyldiacylglycerol,DGDG)在各时间点都持续增长,12h后增长量达26.95%,硫代异鼠李糖甘油二酯(Sulfoquinovosyl diacylglycerol,SQDG)的含量则呈现出不断下降的趋势。研究所建立的脂质组学分析方法对进一步研究Synechocystis sp.PCC 6803的脂质代谢及生理功能提供了有力的分析工具。The photosynthetic glycerolipids composition of cyanobacteria is crucial to the structural and physiological aspects of cells.We developed a method of electrospray ionization tandem mass spectrometry coupled with ultra-performance liquid chromatography to qualitatively and quantitatively analyze the lipidomes of the Synechocystis sp.PCC 6803 cells.Shotgun analysis identified photosynthetic glycerolipids,including lipid classes and fatty acyl composition within lipids,and exhibited high efficiency,selectivity,sensitivity and mass accuracy,Under different light intensities,the content of MGDG and PG increased by 34.64% and 68.49% after 2 hours exposition,respectively.The MGDG and PG molecules containing de novo synthesized saturated fatty acids increased more rapidly and drastically than highly unsaturated species.The content of DGDG increased by 26.95% after 12 hours under high light,while the content of SQDG gradually declined.The method established in this study provides a powerful analytical tool for dissecting metabolism and physiological functions of glycerolipids in cyanobacteria.

关 键 词：脂质组 集胞藻Synechocystis sp.PCC 6803 高光适应 甘油酯 

分 类 号：Q946.4[生物学—植物学]