大鼠颅骨材料力学和骨质参数的增龄性变化  

Age-Related Changes of Material Mechanics and Bone Material Parameters of Rat Skulls

作　　者：李曼 李明杰 黄江[1] 乐翠云[1] 王尧[1] 赵令强 王承飞 万昌武[1] 汪家文[1] LI Man;LI Ming-jie;HUANG Jiang;LE Cui-yun;WANG Yao;ZHAO Ling-qiang;WANG Chengfei;WAN Chang-wu;WANG Jia-wen(School of Forensic Medicine,Guizhou Medical University,Guiyang 550004,China;Department of Pa-thology,First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)

机构地区：[1]贵州医科大学法医学院,贵州贵阳550004 [2]广西医科大学第一附属医院,广西南宁530021

出　　处：《法医学杂志》2021年第1期1-6,共6页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(81660309);贵州省科技厅基金资助项目(黔科合平台人才[2018]5779-3);贵州省高等学校教学内容和课程体系改革资助项目(2019040);贵州省大学生创新创业训练计划资助项目(20195200922,20195200138)。

摘　　要：目的通过检测大鼠颅骨材料力学和骨质参数,探讨二者关系及其与年龄的相关性。方法48只健康雄性SD大鼠按年龄分为2、4、6、8、17、26、52和104周龄组,每组6只。利用KDⅡ-0.2型微机控制电子万能试验机压缩右侧颅盖骨检测其材料力学参数(极限载荷、压缩强度、压缩模量),之后分离颅骨骨片进行Micro-CT系统扫描,检测骨质参数(颅骨厚度、骨密度、骨体积、骨小梁厚度)。结果各组大鼠颅骨极限载荷、压缩强度和压缩模量组间差异均具有统计学意义(P<0.05),在26周范围内与年龄呈正相关(P<0.05)。大鼠颅骨厚度、骨密度、骨体积和骨小梁厚度组间差异均具有统计学意义(P<0.05),在52周内与年龄均呈正相关(P<0.05)。各材料力学参数与骨质参数呈正相关(P<0.05)。结论大鼠骨质参数(颅骨厚度、骨密度、骨体积、骨小梁厚度)和颅骨材料力学参数(极限载荷、压缩强度、压缩模量)在一定范围内与年龄呈正相关关系,可用于年龄推断。Objective To study the relationship between material mechanics and bone material parameters of rat skulls and their correlation with age by examination of the parameters.Methods Forty-eight healthy male SD rats were divided into 2,4,6,8,17,26,52 and 104 week groups according to their age.Each group had six rats.The right cranium was compressed by KD Ⅱ-0.2 microcomputer controlled electronic universal testing machine,and material mechanics parameters(ultimate load,compression strength and compression modulus)were measured,then the skull slices were cut off and scanned by Micro-CT system to detect bone material parameters(skull thickness,bone mineral density,bone volume,and trabecular thickness).Results The differences in ultimate load,compression strength and compression modulus among all groups had statistical significance(P<0.05),and were positively correlated with age within 26 weeks(P<0.05).The differences in skull thickness,bone mineral density,bone volume and trabecular thickness among all groups had statistical significance(P<0.05),and were positively correlated with age within 52 weeks(P<0.05).All material mechanics parameters were positively correlated with bone material parameters(P<0.05).Conclusion There is a positive correlation between bone material parameters(skull thickness,bone mineral density,bone volume,trabecular thickness),material mechanics parameter(skull ultimate load,compression strength,compression modulus)and age in a certain range,which can be used to infer age.

关 键 词：法医病理学 生物力学 年龄 颅骨 材料力学参数 骨质参数 大鼠 

分 类 号：DF795.1[医药卫生—法医学]