基于网络药理学探讨山药治疗糖尿病心肌病的作用机制  

Mechanism of Dioscoreae Rhizoma in Treating Diabetic Cardiomyopathy Based on Network Pharmacology

作　　者：黄楠楠[1] 王锴华 陈珊 孙爽[1] 杨德强[1] 张春晶[1] 都晓伟[1] HUANG Nannan;WANG Kaihua;CHEN Shan;SUN Shuang;YANG Deqiang;ZHANG Chunjing;DU Xiaowei(Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药信息》2021年第3期48-54,共7页Information on Traditional Chinese Medicine

基　　金：国家自然青年科学基金项目(81803985);黑龙江省中医药管理局项目(ZHY18-046);黑龙江中医药大学教育教学研究项目(XJJY2018018)。

摘　　要：目的:采用网络药理学的方法探讨山药治疗糖尿病心肌病的作用机制。方法:在TCMSP数据库中寻找山药的活性成分,并对其进行靶点预测;在疾病数据库检索糖尿病心肌病(DCM)的靶点;利用Venn图得到山药和DCM的共同靶点;使用Cytoscape软件绘制“山药-活性成分-疾病-靶点”网络图;在STRING数据库中进行蛋白互作分析(PPI);利用DAVID数据库进行KEGG通路分析和GO生物功能注释。结果:发现了符合标准的16个活性物质和69个作用靶点,主要涉及ADRB2、ADRB1、NOS3等。在DAVID数据库分析中,获得了63个主要通路和130条GO生物功能注释。结论:山药可能通过多种成分、多个靶点、多条通路来治疗糖尿病心肌病,其作用机理可能是通过主要活性成分(-)-花旗松素和薯蓣皂苷元参与cGMP-PKG通路来调节氧化还原酶活性、凋亡过程的负调控等过程诱导高糖环境下的心肌细胞凋亡,从而防止心肌细胞肥大,进而治疗糖尿病心肌病。Objective:To explore the mechanism of Dioscoreae Rhizoma in treating diabetic cardiomyopathy based on network pharmacology.Methods:The active ingredients of Dioscoreae Rhizoma were found in TCMSP database and its target was predicted.The target of diabetic cardiomyopathy(DCM)was retrieved in disease database;the common target of Dioscoreae Rhizoma and DCM were obtained by the Venn diagram;network diagram of Dioscoreae Rhizoma-active ingredients-disease-target was drawn by using Cytoscape software.Protein-protein interaction(PPI)was analyzed in STRING database and KEGG pathway analysis and GO biological function were annotated by using DAVID database.Results:16 active substances and 69 targets were found,which mainly involved ADRB2,ADRB1,NOS3 and so on.In DAVID s database analysis,63 main pathways and 130 GO biological function annotations were obtained.Conclusion:Dioscoreae Rhizoma may be used to treat DCM through multiple ingredients,multiple targets and multiple pathways.The mechanism of action may be through the main active ingredients(-)-Taxifolin and Diosgenin participating in the cGMP-PKG pathway to regulate oxidoreductase activity,negative regulation of apoptosis process and other processes to induce cardiomyocyte apoptosis under high glucose environment,thereby preventing cardiomyocyte hypertrophy in the treatment of DCM.

关 键 词：网络药理学 糖尿病心肌病 山药 薯蓣皂苷元 靶点 作用机制 

分 类 号：R285[医药卫生—中药学]