响应面法优化金鉴灵软膏成型性工艺及其对小鼠湿疹的影响  

Optimization of Jinjianling Ointment forming process and its effect on eczema in mice by response surface methodology

作　　者：沈颖昕 李宁[1] 张荣榕 范琳 韩荣欣 严铭铭 赵大庆 SHEN Ying-xin;LI Ning;ZHANG Rong-rong;FAN Lin;HAN Rong-xin;YAN Ming-ming;ZHAO Da-qing(Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学,长春130117 [2]长春中医药大学创新实践中心,长春130117

出　　处：《中华中医药杂志》2021年第2期1083-1087,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重点研发计划(No.2017YFC1702100);吉林省科技发展计划项目(No.20180623041TC);长春市科技计划项目(No.17YJ007)。

摘　　要：目的:采用Box-Behnken响应面法对金鉴灵软膏剂的成型性工艺进行优化及验证,并考察其对湿疹的影响。方法:以药物对皮肤的刺激性为评价指标,确定基质种类为蜂蜡;以稳定性实验与均匀性实验结果为评价指标;以麻油与基质比例、融化温度、加热时间为影响因素进行响应面优化设计筛选出最佳成型性工艺;对小鼠进行湿疹造模后,以其耳肿胀度、细胞因子水平及炎症因子病理情况等指标考察最佳成型性工艺下制备出的不同剂量组的样品对小鼠湿疹的疗效。结果:最优成型性工艺为:基质种类为蜂蜡,麻油与基质比例为7.5∶1,融化温度为71℃,加热时间为11min;最佳成型性工艺制备的样品可缓解湿疹中出现的炎症症状。结论:通过响应面分析法筛选出的成型性工艺稳定可靠,最佳成型性工艺下制备的样品对小鼠湿疹的治疗效果显著,抗炎作用效果良好。Objective:To optimize and validate the molding process of Jinjianling ointment by Bo-Behnken response surface method,and to investigate the effect of ointment on eczema.Methods:The stimulating effect of the drug on the skin was used as the evaluation index to determine the matrix type as beeswax.The results of stability experiment and uniformity experiment were selected as the evaluation indexes.The response surface optimization was carried out with the ratio of sesame oil to matrix,melting temperature and heating time as the influencing factors.The optimal molding process was designed and screened.After the eczema modeling of mice,the samples of different dosage groups prepared under the optimal molding process were investigated with indicators of ear swelling,cytokine levels and pathological conditions of inflammatory factors.Results:The optimal moldability process was as follows:the matrix type was beeswax,the ratio of sesame oil to substrate was 7.5∶1,the melting temperature was 71°C,and the heating time was 11 min.The samples prepared by the optimal molding process can alleviate the inflammatory symptoms in eczema.Conclusion:The shape-forming process screened by response surface analysis method is stable and reliable,and the sample prepared under the optimal moldability process has significant therapeutic effect on mouse eczema,and the anti-inflammatory effect is promising.

关 键 词：中药软膏 响应面 成型性工艺 湿疹 

分 类 号：R285.5[医药卫生—中药学]