Megsin基因C2093T基因多态性与广西汉族人群IgA肾病中医证型关系的研究  

Relationship between Megsin gene C2093T polymorphism and TCM syndromes of IgA nephropathy in the Guangxi Han population

作　　者：龙春莉[1] 谢永祥[3] 史伟[2] LONG Chun-li;XIE Yong-xiang;SHI Wei(Guangxi University of Chinese Medicine,Nanning 530000,China;The First A ffiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,China)

机构地区：[1]广西中医药大学,南宁530000 [2]广西中医药大学第一附属医院,南宁530023 [3]广西中医药大学第一附属医院肾病科,南宁530023

出　　处：《中华中医药杂志》2021年第1期111-115,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金地区科学基金项目(No.81460719);广西自然科学基金项目(No.2017JJA140492Z);广西名中医史伟传承工作室。

摘　　要：目的:探讨Megsin基因C2093T基因多态性与广西汉族人群IgA肾病(IgAN)患者中医证型的关系。方法:中医将IgAN病例辨证分为脾肾气虚证、肝肾阴虚证、气阴两虚证、风湿热毒证4型。采用PCR-RFLP检测86例IgAN组和59名正常组Megsin基因C2093T位点基因多态性;分析其基因型及等位基因在IgAN病不同中医证型中的分布规律。结果:①Hardy-Weinberg平衡检验:Megsin基因C2093T存在3种突变基因型,即CC型、CT型、TT型及C、T两种等位基因,IgAN组和正常对照组Megsin基因C2093T基因型及等位基因频率均具有群体代表性;②IgAN组和正常组3种突变基因型之间频率比较无显著差异,两组C、T两种等位基因频率分布差异具有统计学意义(P<0.05);③IgAN组中医证型分布规律:脾肾气虚证>气阴两虚证>肝肾阴虚证>风湿热毒证;④IgAN脾肾气虚证组基因型中CC型占比例最大,脾肾气虚证组Megsin基因C2093T基因型与正常对照组比较差异有统计学意义(P<0.05);⑤IgAN各中医证型之间基因型及等位基因频率比较均无明显差异。结论:IgAN患者中医证型以虚证为多,脾肾气虚证及气阴两虚证为其主要证型。Megsin基因C2093T位点基因型CC型可能是IgA肾病脾肾气虚证发病的易感基因。Objective:To investigate the relationship between the polymorphism of the Megsin gene C2093 T and the expression of TCM syndromes in IgA nephropathy patients in Guangxi Han population.Methods:According to TCM syndrome differentiation,IgA nephropathy cases were divided into four types:syndrome of qi deficiency of spleen and kidney,syndrome of yin deficiency of liver and kidney,syndrome of deficiency of both qi and yin and syndrome of wind-dampand and toxic heat.The polymorphism of C2093 T locus of Megsin gene in 86 cases of IgA nephropathy group and 59 cases of normal group was detected by PCR-RFLP and the distribution of its genotype and allele in different TCM syndromes of IgA nephropathy was analyzed.Results:①Hardy-Weinberg balance test showed that there were three mutant genotypes of Megsin gene C2093 T of Megsin gene,namely CC,CT,TT and C,T alleles.The C2093 T genotype and allele frequencies of Megsin gene in IgA nephropathy group and normal group were all representative of population.②There was no significant difference in the frequencies of the three mutant genotypes between IgA nephropathy group and normal group,but the frequency distribution of C and T alleles in two groups had statistical significance(P<0.05).③Distribution regularity of TCM syndromes in IgA nephropathy group:syndrome of qi deficiency of spleen and kidney>syndrome of deficiency of both qi and yin>syndrome of yin deficiency of liver and kidney>syndrome of wind-damp and toxic heat.④The proportion of CC genotype in IgA nephropathy with syndrome of qi deficiency of spleen and kidney group was the largest.The C2093 T genotype of Megsin gene in syndrome of qi deficiency of spleen and kidney group was significantly different from that in normal group(P<0.05).⑤There was no significant difference in genotype and allele frequencies of IgA nephropathy among TCM syndromes.Conclusion:The syndromes of TCM in IgA nephropathy patients are characterized by deficiency syndromes,which the syndrome of qi deficiency of spleen and kidney and the synd

关 键 词：IGA肾病 MEGSIN 多态性 中医证型 

分 类 号：R277.5[医药卫生—中医学]