黑曲霉对不同陈化年份广陈皮黄酮类成分的影响  

Effects of Aspergillus Niger on flavonoids from Guangdong Citri Reticulatae Pericarpium with different aging years

作　　者：杨丹 徐双美 熊素琴 陈鸿平[1] 刘友平[1] YANG Dan;XU Shuang-mei;XIONG Su-qin;CHEN Hong-ping;LIU You-ping(Department of Pharmacy,Chengdu University of TCM,Standardization Education Ministry Key Laboratory of Traditional Chinese Medicine,the Breeding Base of State Key Laboratory of Resources System Research and Development Utilization of Chinese Herbal Medicines Co-constructioned by The Ministry of Science and Technology of the PCR and Sichuan Province,Chengdu 611137,China)

机构地区：[1]成都中医药大学药学院,中药材质量标准化教育部重点实验室,中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137

出　　处：《中华中医药杂志》2021年第1期492-495,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81973436);国家基础科学人才培养基金项目(No.J1310034);四川省科技计划项目(No.2015JY0012);成都中医药大学双一流中药质量保障提升创新科研团队。

摘　　要：目的:考察黑曲霉对不同陈化年份广陈皮黄酮类成分的影响。方法:将黑曲霉反接至无菌广陈皮,恒温恒湿培养,于不同时间点进行取样,采用超高效液相色谱法(UPLC)和紫外分光光度计法测定广陈皮黄酮类成分。结果:黑曲霉反接后,不同陈化年份广陈皮总黄酮、橙皮苷、川陈皮素及橘皮素含量均有提升,且UPLC指纹图谱显示有新成分增加。其中陈化2年黄酮类成分变化较大,除橘皮素含量变化不明显外,其总黄酮、橙皮苷和川陈皮素含量各增长了72.24%、69.93%和72.34%。结论:黑曲霉对不同陈化年份广陈皮的黄酮类成分均有一定影响,影响最大的是陈化2年广陈皮,其中具体的转化机制还有待深究。Objective:To investigate the effects of Aspergillus Niger on the flavonoids of Guangdong Citri Reticulatae Pericarpium(GCRP)in different aging years.Methods:Aspergillus Niger was reversely grafted to germ-free GCRP,incubated at constant temperature and humidity,and sampled at different time points.The flavonoids were determined by ultra-high performance(UPLC)liquid chromatography and ultraviolet spectrophotometer.Results:The contents of total flavonoids,hesperidin,nobiletin and tangerine of GCRP in different aging years were increased after Aspergillus Niger was reverse-grafted.Among them,the content of flavonoids aged for 2 years changed significantly.Except that the content of tangerine did not change significantly,the content of total flavonoids,hesperidin and nobiletin increased by 72.24%,69.93%and 72.34%,respectively.Conclusion:Aspergillus Niger has a certain influence on the flavonoids of GCRP in different aging years,and the biggest influence is aging GCRP in 2 years,but the specific transformation mechanism remains to be further studied.

关 键 词：广陈皮 黑曲霉 黄酮类 陈化年份 超高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]