我国部分地区羊驼十二指肠贾第虫的PCR检测和鉴定  

Detection and identification of Giardia duodenalis in alpaca(Vicugna pacos) by PCR in China

作　　者：黄玫瑰 张齐元 汪文 徐春艳 侯旻昱 王甜[1] 井波[1] Huang Meigui;Zhang Qiyuan;Wang Wen;Xu Chunyan;Hou Minyu;Wang Tian;Jing Bo(Engineering Laboratory of Tarim Animal Disease Diagnosis and Control,Xinjiang Production and Construction Corps,College of Animal Science,Tarim University,Xinjiang Alar 843300;Aksu Vocational and Technical College,Xinjiang Aksu 843000)

机构地区：[1]塔里木大学动物科学学院,新疆生产建设兵团塔里木动物疫病诊断与防控工程实验室,新疆阿拉尔843300 [2]阿克苏职业技术学院,新疆阿克苏843000

出　　处：《现代畜牧兽医》2021年第3期53-55,共3页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：国家自然科学基金地区基金(31860699);新疆生产建设兵团重点领域科技攻关计划项目(2020AB025);塔里木大学校长基金(TDZKSS201907)。

摘　　要：为了解我国羊驼十二指肠贾第虫的感染情况,收集我国9个县/市的羊驼养殖基地共484份羊驼粪便样本,提取DNA后,基于核糖体小亚基(SSU rRNA)基因位点对其进行PCR扩增。结果表明:8份样本呈十二指肠贾第虫PCR检测阳性,总感染率为1.7%;9个养殖基地中,6个呈十二指肠贾第虫感染阳性。对成功测序的8条序列进行比对分析,鉴定出2种人兽共患集聚体为集聚体A (n=5)和集聚体B (n=3)。研究表明,我国部分地区羊驼十二指肠贾第虫感染率较低,但存在人兽共患集聚体。To understand the infection of G. duodenalis in alpaca in China, a total of 484 faecal samples from alpaca in nine counties/cities in China were collected, and all DNA were detected by PCR based on the small ribosomal subunit(SSU rRNA) gene of G. duodenalis. Eight samples were positive for G. duodenalis, with the infection rate was 1.7%.Among of nine collection sites, six were positive for G. duodenalis. Eight sequences of G. duodenalis were analyzed, with two zoonotic assemblage were identified assemblage A(n=5) and assemblage B(n=3). Research showed that the infection rate of G. duodenalis in alpaca was low in China, however, alpacas maybe played a zoonotic role of G. duodenalis assemblage A and assemblage B.

关 键 词：十二指肠贾第虫 感染 检测 鉴定 羊驼 

分 类 号：S855.9[农业科学—临床兽医学]