HPLC法同时测定气管炎丸中6个活性成分的含量  

Simultaneous determination of six active components in Qiguanyan pills by HPLC

作　　者：郑佳[1] 林海霞 ZHENG Jia;LIN Hai-xia(Leshan Vocational and Technical College,Leshan 614000,China;Leshan Food and Dmg Inspection Center,Leshan 614000,China)

机构地区：[1]乐山职业技术学院,乐山614000 [2]乐山市食品药品检验检测中心,乐山614000

出　　处：《药物分析杂志》2021年第2期219-225,共7页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立HPLC法同时测定气管炎丸中绿原酸、芍药苷、柚皮苷、黄芩苷、黄芩素、汉黄芩素的含量。方法:采用Agilent 5 TC-C_(18)(2)(250 mm×4.6 mm,5μm)色谱柱,以乙腈(A)-0.2%磷酸水溶液(B)为流动相,梯度洗脱(0~50 min,2%A→34%A;50~61 min,34%A→55%A;61~62 min,55%A→2%A;62~66 min,2%A),流速1.0 mL·min^(-1),柱温30℃,检测波长分别为327 nm(绿原酸)、230 nm(芍药苷)和280 nm(柚皮苷、黄芩苷、黄芩素、汉黄芩素),进样体积10μL。结果:6个活性成分在相应浓度范围内线性关系良好(r>0.999);绿原酸、芍药苷、柚皮苷、黄芩苷、黄芩素、汉黄芩素的平均回收率分别为101.0%(RSD=1.3%)、99.2%(RSD=1.1%)、98.3%(RSD=1.4%)、99.9%(RSD=1.3%)、101.2%(RSD=1.3%)、101.5%(RSD=1.3%);精密度、稳定性、重复性良好,RSD均小于2%。含量测定结果分别为0.2959~0.3758、0.7114~0.8184、0.3614~0.4892、1.169~1.555、0.03576~0.05287、0.1021~0.1675 mg·g^(-1)。结论:该方法简单、准确、快速,可为气管炎丸后续质量标准的提高提供实验参考。Objective:To establish a method for the simultaneous determination of chlorogenic acid,paeoniflorin,naringin,baicalin,baicalein and wogonin in Qiguanyan pills.Methods:HPLC analysis was performed on the column of Agilent 5 TC-C_(18)(2)(250 mm×4.6 mm,5μm)with the mobile phase of acetonitrile-0.2%phosphoric acid at a flow rate of 1.0 mL·min^(-1),the column temperature was 30℃,and the detection wavelength was 327 nm for chlorogenic acid,230 nm for paeoniflorin and 280 nm for naringin,baicalin,baicalein,wogonin,and the volume injection was 10μL.Results:Six active components showed good linear relationships within their own ranges(r>0.999),the average recoveries were 101.0%(RSD=1.3%),99.2%(RSD=1.1%),98.3%(RSD=1.4%),99.9%(RSD=1.3%),101.2%(RSD=1.3%)and 101.5%(RSD=1.3%)respectively.The precision,stability,and repeatability were good with RSDs less than 2%.The content determination results of chlorogenic acid,paeoniflorin,naringin,baicalin,baicalein and wogonin were 0.2959-0.3758,0.7114-0.8184,0.3614-0.4892,1.169-1.555,0.03576-0.05287,0.1021-0.1675 mg·g^(-1),respectively.Conclusion:The established method is simple,rapid,accurate and can be used for improvement of subsequent quality standards of Qiguanyan pills.

关 键 词：气管炎丸 高效液相色谱法 绿原酸 芍药苷 柚皮苷 黄芩苷 黄芩素 汉黄芩素 

分 类 号：R917[医药卫生—药物分析学]