新西兰匍柄霉(Stemphylium eturmiunum)HSP42基因沉默突变株的构建及其生物学特性  

Construction and Biological Characteristics of HSP42 Gene Silencing Mutant from Stemphylium eturmiunum

作　　者：万能 王群[2] 王石[2] 徐文梦 赵丽丽 孙文秀[1] 张修国[2] 李伟[1] WAN Neng;WANG Qun;WANG Shi;XU Wen-meng;ZHAO Li-li;SUN Wen-xiu;ZHANG Xiu-guo;LI Wei(College of Life Science/Yangtze University,Jingzhou 434025,China;College of Plant Protection/Shandong Agricultural University,Key Laboratory of Vegetable Pest and Biology of Shandong Province,Tai’an 271018,China)

机构地区：[1]长江大学生命科学学院,湖北荆州434025 [2]山东农业大学植物保护学院,山东省蔬菜病虫生物学省级重点实验室,山东泰安271018

出　　处：《山东农业大学学报（自然科学版）》2021年第1期1-6,共6页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：国家大宗蔬菜产业技术体系(CARS-25-03B)。

摘　　要：丝孢真菌是自然界中一类广泛存在的真核微生物,匍柄霉属(Stemphylium)是丝孢真菌中的一个重要类群,而新西兰匍柄霉(Stemphylium eturmiunum)是其中的典型种,是研究丝孢真菌有性、无性进化的适用材料。热休克蛋白(Heat shock protein,HSP),是一类进化保守的蛋白质家族,在生物体中发挥着重要的生理功能。本研究以S.eturmiunum为实验材料,克隆鉴定了热休克蛋白42(HSP42)基因,通过根癌农杆菌介导的匍柄霉属遗传转化系统,获得了HSP42(03717)基因沉默菌株。以S.eturmiunum WT菌株为对照,对03717沉默菌株的生物学特性进行观察发现:沉默03717后,其菌落生长减缓,菌丝细胞核分布不均匀;沉默03717后其产分生孢子时间推迟,产孢量减少,且经低温诱导后无法产生子囊壳及子囊孢子,说明HSP42基因对S.eturmiunum的无性和有性发育具有重要影响。本研究为S.eturmiunum有性无性发育及小热休克蛋白功能研究提供了更多依据。Filamentous fungi is a kind of eukaryote which exists widely in nature,the Stemphylium is an important group of filamentous fungi,and the Stemphylium eturmiunum is a typical species,is a suitable material for studying sexual and asexual evolution of filamentous fungi.Heat shock protein(HSP),a class of evolutionarily conserved protein families,plays an important physiological function in organisms.The heat shock protein 42(HSP42)gene was cloned and identified by S.eturmiunum as the experimental material,and the HSP42(03717)gene silencing strain was obtained by Agrobacterium tumefaciens mediated genetic transformation system of Trichoderma.Taking S.eturmiunum WT strain as control,the biological characteristics of 03717 silent strain were observed:the colony growth slowed down and the mycelium nucleus distribution was uneven after silencing 03717;after silencing 03717,the conidia production time was delayed and the perithecia and ascospore could not be produced after low temperature induction.It shows that heat shock protein 42 gene has important effect on asexual and sexual development of S.eturmiunum.For S.eturmiunum sexual development and small heat shock protein function,this study provides more evidence.

关 键 词：新西兰匍柄霉 热休克蛋白 基因沉默 

分 类 号：S763.15[农业科学—森林保护学]