杂交组合断奶仔猪腹泻与FUT1基因遗传变异的关联分析  

Analysis of Correlation between Diarrhea and Genetic Variation of FUT1 Gene in Weaned Piglets of Hybrid Combination

作　　者：方晨 郭飞 胡瑞举 杨明华[1] 张斌 刘韶娜 黄英[1] 赵彦光 赵素梅[1] FANG Chen;GUO Fei;HU Ruiju;YANG Minghua;ZHANG Bin;LIU Shaona;HUANG Ying;ZHAO Yanguang;ZHAO Sumei(Yunnan Key Laboratory of Animal Nutrition and Feed,Yunnan Agricultural University,Kunming 650201,China;Yunnan Animal Science and Veterinary Institute,Kunming 650224,China)

机构地区：[1]云南农业大学云南省动物营养与饲料重点实验室,昆明650201 [2]云南省畜牧兽医科学院,昆明650224

出　　处：《畜牧兽医学报》2021年第3期610-619,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31760645,31260592,31060331);云南省重点研发项目(2018BB003);云南省省级财政项目(20151105562255);云南省教育厅科学研究基金项目(2019Y0086);云南省应用基础研究面上项目(2017FB068);科技创新人才计划(2020FA011);州校合作项目(2020ZXND02)。

摘　　要：旨在探究杂交组合断奶仔猪腹泻与α(1,2)岩藻糖转移酶基因1(FUT1)遗传变异的关联性。本试验选择35日龄的杜滇、杜藏、杜洛克断奶仔猪为研究对象,按品种分为3组,分别为113、165、87个重复。通过测序对FUT1基因多态性进行检测,分析基因多态性与断奶仔猪腹泻率的相关性。结果发现,FUT1基因存在T18C、C229T、C2418A、A306G 4个多态性位点,多态性和杂合度均处于中度,C229T、C2418A、A306G为错义突变,可能是影响猪腹泻的关键位点。T18C位点TT基因型的杂交组合杜滇猪和杜藏猪、C229T位点的CC基因型、C2418A位点的CA基因型和A306G的GG基因型的3种猪腹泻程度相对较严重,呈显性遗传。结果说明,品种和FUT1基因多态性对断奶仔猪腹泻的影响有一定的差异性和相关性。The study aimed to explore the relationship between diarrhea of weaned piglets of hybrid combination and genetic variation of α-1,2-fucosyltransferase 1(FUT1)gene.In this experiment,35-day-old Dudian,Duzang and Duroc weaned piglets were selected as the research objects,and were divided into 3 groups according to breeds,with 113,165 and 87 replicates,respectively.FUT1 gene polymorphism was detected by sequencing to analyze the correlation between gene polymorphism and diarrhea rate of weaned piglets.The results showed that there were 4 polymorphic sites in FUT1 gene,including T18C,C229T,C2418A and A306G,all of which were in moderate polymorphism and heterozygosity.C229T,C2418A and A306G were missense mutations,which might be key sites affecting porcine diarrhea.The degree of diarrhea was relatively serious in the hybrid combination Dudian,Duzang pig with TT genotype at T18C,and 3 pig populations with CC genotype at C229T,CA genotype at C2418A and GG genotype at A306G,which showed dominant inheritance.The result suggest that the effects of breeds and FUT1 gene polymorphisms on weaned piglets diarrhea have certain differences and correlations.

关 键 词：杂交组合 FUT1基因 遗传变异 断奶仔猪 腹泻 抗病育种 

分 类 号：S828.2[农业科学—畜牧学]