m^(6)A去甲基化酶ALKBH5与弱精症的相关性研究  被引量:4

Correlation between ALKBH5 and asthenozoospermia

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作  者:夏维 胡玲[2] 刘东[3] 刘琴[4] 胡红兵 XIA Wei;HU Ling;LIU Dong;LIU Qin;HU Hongbin(Department of Clinical Laboratory,Wuhan Children′s Hospital/Wuhan Maternal and Child Healthcare Hospital,Tongji Medical College,Huazhong University of Science&Technology,Wuhan,Hubei 430015,China;Department of Emergency,Wuhan Children′s Hospital/Wuhan Maternal and Child Healthcare Hospital,Tongji Medical College,Huazhong University of Science&Technology,Wuhan,Hubei 430015,China;Operating Room,Wuhan Children′s Hospital/Wuhan Maternal and Child Healthcare Hospital,Tongji Medical College,Huazhong University of Science&Technology,Wuhan,Hubei 430015,China;Department of Blood Transfusion,Wuhan Children′s Hospital/Wuhan Maternal and Child Healthcare Hospital,Tongji Medical College,Huazhong University of Science&Technology,Wuhan,Hubei 430015,China)

机构地区:[1]华中科技大学同济医学院附属武汉儿童医院/武汉市妇幼保健院血液肿瘤科,湖北武汉430015 [2]华中科技大学同济医学院附属武汉儿童医院/武汉市妇幼保健院急诊科,湖北武汉430015 [3]华中科技大学同济医学院附属武汉儿童医院/武汉市妇幼保健院手术室,湖北武汉430015 [4]华中科技大学同济医学院附属武汉儿童医院/武汉市妇幼保健院输血科,湖北武汉430015

出  处:《国际检验医学杂志》2021年第6期700-703,共4页International Journal of Laboratory Medicine

基  金:武汉市临床医学科研项目(WX15C22)。

摘  要:目的探究N^(6)-甲基腺嘌呤(m^(6)A)去甲基化酶ALKBH5与弱精症的相关性。方法收集精液标本30份,包括弱精症患者与健康者精液标本各15份,密度梯度离心纯化精子,使用液相色谱与质谱联用(LC-MS/MS)检测精子RNA的m^(6)A水平,使用实时荧光定量PCR法检测ALKBH5相对表达水平。结果弱精症患者m^(6)A水平明显高于健康者,ALKBH5相对表达水平低于健康者,差异均有统计学意义(P<0.05)。精子活动度参数相关性分析结果显示,ALKBH5相对表达水平与前向运动精子数和非前向运动精子数呈正相关(r=0.512、0.377,P<0.05),与不动精子数呈负相关(r=-0.497,P<0.05);m^(6)A与前向运动精子数和非前向运动精子数呈负相关(r=-0.442、-0.425,P<0.05),与不动精子数呈正相关(r=0.528,P<0.05)。结论在弱精症患者中,m^(6)A及其去甲基化酶ALKBH5与精子活动度具有相关性。Objective To investigate the correlation between N^(6)-methyladenosine(m^(6)A)demethylase ALKBH5,and asthenozoospermia.Methods Semen samples from 15 asthenozoospermia patients and 15 healthy controls were collected.The sperm was purified by density gradient centrifugation.m^(6)A contents in sperm RNA was detected by Liquid Chromatograph Mass Spectrometer(LC-MS/MS),and mRNA expression of ALKBH5 was determined by qPCR.Results m^(6)A level in asthenozoospermia patients was significantly higher than that of healthy controls,and the mRNA level of ALKBH5 was significantly higher than that of healthy controls,and the differences were statistically significant(P<0.05).ALKBH5 was positively correlated with progressive motility and non-progressive motility(r=0.512,0.377,P<0.05),and was negatively correlated with immotility(r=-0.497,P<0.05).m^(6)A was negatively correlated with progressive motility and non-progressive motility(r=-0.422,-0.425,P<0.05)and was positively correlated with immotility(r=0.528,P<0.05).Conclusion The results suggest that m^(6)A and demethylase ALKBH5 are correlate with sperm motility.

关 键 词:ALKBH5 N^(6)-甲基腺嘌呤 弱精症 精子活动度 

分 类 号:R446.19[医药卫生—诊断学]

 

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