抗心律失常药对截肢大鼠炎性因子表达及心肌电活动的影响  

Effects of antiarrhythmic drugs on the expression of inflammatory factors and the electrical activity of myocardium in amputated rats

作　　者：赵桂香[1] 刘佳[1] ZHAO Guixiang;LIU Jia(Department of Cardiology,the Sixth Affiliated Hospital,Xinjiang Medical University,Urumuqi 830000,China)

机构地区：[1]新疆医科大学第六附属医院心血管内科,乌鲁木齐830000

出　　处：《国际心血管病杂志》2021年第2期112-115,128,共5页International Journal of Cardiovascular Disease

基　　金：新疆维吾尔自治区自然科学基金资助项目(2018D01C315)。

摘　　要：目的:观察抗心律失常药对截肢大鼠炎性因子表达及心肌电活动的影响。方法:选取30只健康清洁级雄性SD大鼠,随机分为A组、B组、C组、D组和E组,每组6只,建立左后肢截肢手术创伤模型,其中A组给予生理盐水,B组、C组、D组和E组分别给予利多卡因(2 mg/kg)、普萘洛尔(7 mg/kg)、胺碘酮(200 mg/kg)和维拉帕米(20 mg/kg),记录并比较手术前后大鼠心率、QT间期、PR间期、单相动作电位(MAP)振幅、左室收缩压(LVSP)变化,及肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和C反应蛋白(CRP)水平。结果:C组、D组和E组术后15 min、30 min、60 min时心率明显低于A组和B组(P<0.05);B组、C组、D组和E组术后15 min、30 min、60 min和120 min时PR间期、QT间期高于A组(P<0.05);B组术后15 min、30 min、60 min和120 min时MAP振幅明显低于A组、C组、D组和E组(P<0.05);B组、C组、D组和E组术后15 min、30 min、60 min和120 min时LVSP明显低于A组(P<0.05);A组、B组、C组、D组和E组术后2 h时血清TNF-α、IL-6和CRP比较差异均无统计学意义。结论:抗心律失常药有助于减轻截肢创伤对大鼠心肌电活动的影响,但对炎性因子水平无明显作用。Objective:To investigate the effect of antiarrhythmic drugs on the expression of inflammatory factors and the electrical activity of myocardium in amputated rats.Methods:A total of 30 healthy and clean-grade male SD rats were randomly divided into group A,group B,group C,group D and group E,with 6 rats in each group.The model of left hindlimb amputation injury was established in each group.The rats in the group A were given normal saline,and rats in the group B,group C,group D and group E were given lidocaine(2 mg/kg),propranolol(7 mg/kg),amiodarone(200 mg/kg)and verapamil(20 mg/kg),respectively.The heart rate,QT interval,PR interval,monophasic action potential(MAP)amplitude,left ventricular systolic pressure(LVSP),and the level of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and C-reactive protein(CRP)were measured before and after the operation.Results:The heart rate of group C,group D and group E at 15 min,30 min and 60 min after surgery were significantly lower than that of group A and group B(all P<0.05).The PR interval and QT interval of group B,group C,group D and group E at 15 min,30 min,60 min and 120 min after surgery were higher than that of group A(all P<0.05).The amplitude of MAP at 15 min,30 min,60 min and 120 min after surgery in group B was significantly lower than that in group A,group C,group D and group E(all P<0.05).In group B,group C,group D and group E,LVSP at 15 min,30 min,60 min and 120 min after surgery was significantly lower than that in group A(all P<0.05).There was no statistically significant difference in level of serum TNF-α,IL-6 and CRP between group A,group B,group C,group D and group E at 2 hours after surgery(all P>0.05).Conclusions:Antiarrhythmic drugs could reduce the effect of amputation on the myocardial electrical activity in rats,but have no obvious effect on the expression of inflammatory factors.

关 键 词：抗心律失常药 截肢大鼠 炎性因子 心肌电活动 

分 类 号：R965[医药卫生—药理学]