柴胡和白芍醋炙前后组方四逆散对抑郁大鼠粪便代谢组学的比较  

A Comparative Study on Fecal Metabonomics in Depressed Rats Intervened by Sinisan Containing Bupleuri Radix and Paeoniae Radix Alba before and after Vinegar-Processing

作　　者：莫子晴 蔡皓[1] 段煜[1] 裴科 张雅婷 钮敏洁 王梦晴 于慧 MO Zi-qing;CAI Hao;DUAN Yu;PEI Ke;ZHANG Ya-ting;NIU Min-jie;WANG Meng-qing;YU Hui(School of Pharmacy, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, 210023, China;Institute of Pharmaceutical and Food Engineering, Shanxi University of Traditional Chinese Medicine, Taiyuan, 030024, China)

机构地区：[1]南京中医药大学药学院,国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210023 [2]山西中医药大学制药与食品工程学院,山西太原030024

出　　处：《南京中医药大学学报》2021年第2期216-224,共9页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81673600);江苏省研究生科研创新计划(KYCX18_1637)。

摘　　要：目的应用1H-NMR代谢组学技术探究慢性不可预知温和应激(CUMS)抑郁大鼠粪便代谢组学的变化,评价柴胡和白芍醋炙前后组方四逆散的抗抑郁作用,并探讨四逆散中君药柴胡和臣药白芍醋炙的增效机制及炮制内涵。方法利用CUMS程序建立大鼠抑郁模型,以1H-NMR为技术手段研究不同给药情况下抑郁大鼠粪便代谢组学的变化。结果CUMS抑郁大鼠模型复制成功。代谢组学结果表明,与空白组比较,模型组大鼠中共有17种代谢物水平发生变化。与模型组比较,四逆散组及柴胡和白芍醋炙后组方的四逆散组干预后的抑郁大鼠代谢发生显著变化。与四逆散组比较,柴胡和白芍醋炙后组方的四逆散组干预的抑郁大鼠粪便中乳酸、α-葡萄糖、β-葡萄糖、甲酸、乙酸、苏氨酸6种代谢物的水平发生变化,同时对丙酮酸代谢、糖酵解与糖质新生代谢、甲烷代谢、乙醛酸和二羧酸代谢、甘氨酸-丝氨酸-苏氨酸代谢、氨酰合成代谢共6条代谢通路产生影响。结论柴胡和白芍醋炙后组方的四逆散抗抑郁作用更佳,这可能与能量代谢、氨基酸代谢、免疫调控及肠道菌群有关。OBJECTIVE To explore the changes of fecal metabonomics in the depressed rats with chronic unpredictable mild stress(CUMS)by 1H-NMR metabonomics technology,evaluate the antidepressant effects of Sinisan containing Bupleuri Radix and Paeoniae Radix Alba before and after vinegar-processing,and discuss the effect enhancement mechanism and processing connotation of Sinisan containing vinegar-processed Bupleuri Radix and vinegar-processed Paeoniae Radix Alba.METHODS The depressed rat model was established using the CUMS program,and the changes of fecal metabonomics in depressed rats under different administration conditions were investigated by 1H-NMR technology.RESULTS The CUMS depressed rat model was successfully replicated.The results of metabonomics showed that,compared to the control group,a total of 17 metabolites were changed in the rats of the model group.Compared to the model group,there were significant changes in the depressed rats intervened by Sinisan and Sinisan containing Bupleuri Radix and Paeoniae Radix Alba after vinegar-processing.Compared to the Sinisan group,a total of 6 metabolites,including lactic acid,α-glucose,β-glucose,formic acid,acetic acid,and threonine,were changed in the feces of the rats intervened by Sinisan containing Bupleuri Radix and Paeoniae Radix Alba after vinegar-processing.Meanwhile,a total of 6 metabolic pathways,including pyruvate metabolism,glycolysis or gluconeogenesis,methane metabolism,glyoxylate and dicarboxylate metabolism,glycine,serine and threonine metabolism,aminoacyl-tRNA biosynthesis,were influenced as well.CONCLUSION Sinisan containing Bupleuri Radix and Paeoniae Radix Alba after vinegar-processing shows better antidepressant effect,which may be related to energy metabolism,amino acid metabolism,immune regulation,and intestinal flora.

关 键 词：柴胡 白芍 醋炙 四逆散 抑郁症 代谢组学 

分 类 号：R285.5[医药卫生—中药学]