植物三萜类成分生物合成中氧鲨烯环化酶与细胞色素P450的研究进展  

Advances in Research on the Oxidosqualene Cyclases and Cytochrome P450 Involved in Plant Triterpene Biosynthesis

作　　者：田荣 谷巍[1,2] 韦陈彬 徐飞 邱蓉丽[1] 张阿琴[1] 季媛媛 李桃 TIAN Rong;GU Wei;WEI Chen-bin;XU Fei;QIU Rong-li;ZHANG A-qin;JI Yuan-yuan;LI Tao(School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing, 210023, China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏省中药资源产业化过程协同创新中心,江苏南京210023

出　　处：《南京中医药大学学报》2021年第2期303-310,共8页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(82073958,81673534);中医药公共卫生服务补助专项(财社〔2018〕43号);医疗服务与保障能力提升补助资金(中医药事业传承与发展部分)(财社〔2019〕39号);江苏省中药资源产业化过程协同创新中心项目(ZDXM-3-24)。

摘　　要：三萜是许多药用植物的活性成分,由于结构相对复杂,植物中含量较低,通过提取分离或化学合成难以获得,其应用受到限制。目前对三萜类成分的生物合成研究已取得一定的进展,其中氧鲨烯环化酶(OSC)可催化2,3-氧化鲨烯环化生成三萜类前体物质,细胞色素P450(CYP450)主要参与三萜的后修饰过程,对三萜的多样性起着关键作用。通过OSC和CYP450在三萜生物合成中的催化功能的综述,以期为进一步探索OSC和CYP450在三萜生物合成中的功能研究提供思路。Triterpenes,the active ingredients of many medicinal plants,are difficult to be extracted,separated or chemical synthesized because of their complex structures and low amount in plants.Thus,the application of triterpenes is extensively limited.Until now,progress on the triterpene biosynthesis pathway has been made to some progress.Notably,oxidosqualene cyclase(OSC)can catalyze 2,3-oxidosqualene cyclization to generate precursors of triterpene.And cytochrome P450(CYP450)is mainly involved in the post-modification process,playing a key role in the diversity of triterpenes.The aim of present study is to provide ideas for the functional research of key enzymes in triterpene biosynthesis by reviewing the researches about the catalytic function of OSC and CYP450 in triterpene biosynthesis.

关 键 词：三萜类成分 氧鲨烯环化酶 细胞色素P450 

分 类 号：R282.71[医药卫生—中药学]