光照度对蒲公英光合效率及主要黄酮类化合物含量的影响  

Effect of illuminance on photosynthetic efficiency and main flavonoids content in Taraxacum mongolicum

作　　者：徐翌深 谢小翌 郭仰东[2] 张喜春[1] XU Yi-shen;XIE Xiao-yi;GUO Yang-dong;ZHANG Xi-chun(School of Plant Science and Technology,Beijing Agricultural College,Beijing 102206,China;College of Horticulture,China Agricultural University,Beijing 100083,China)

机构地区：[1]北京农学院植物科学技术学院,北京102206 [2]中国农业大学园艺学院,北京100083

出　　处：《湖北农业科学》2021年第5期85-88,共4页Hubei Agricultural Sciences

基　　金：都市绿色农业蔬菜新品种选育项目(2023299007)。

摘　　要：为探究光照度与蒲公英(Taraxacum mongolicum Hand.-Mazz.)光合速率及5种主要黄酮类化合物含量的关系,在蒲公英生长过程中进行5种不同光照度处理,透光率分别为100.0%(平均光照度15882 lx)、82.8%(平均光照度13116 lx)、60.3%(平均光照度9545 lx)、37.9%(平均光照度6003 lx)、14.5%(平均光照度2303 lx),采用植物光合作用测定仪和高效液相色谱法(HPLC)测定样品的光合参数和5种主要黄酮类化合物含量。结果表明,5种光照处理中,对照组(透光率100%)蒲公英光合效率最佳,透光率60.3%处理下5种黄酮类化合物含量最高,分别为芦丁346.85μg/g、槲皮素502.75μg/g、木犀草素299.53μg/g、山奈酚241.90μg/g、异鼠李素273.35μg/g。栽培蒲公英过程中,全光照条件下光合效率最高;透光率控制在60.3%左右最有利于其黄酮类化合物的积累,低于60.3%不利于其生长。Aiming to investigate the relationship among illuminance and photosynthetic efficiency and accumulation of five main flavonoids in Taraxacum mongolicum Hand.-Mazz.,five different light intensities treatments were applied including light transmittance 100.0%(average illuminance 15882 lx),82.8%(average illuminance 13116 lx),60.3%(average illuminance 9545 lx),37.9%(average illuminance 6003 lx)and 14.5%(average illuminance 2303 lx)during the growth of Taraxacum mongolicum,then the photosynthetic parameters and the content of five main flavonoids were determined by plant photosynthesis analyzer and high performance liquid chromatography(HPLC).The results showed that,the highest accumulation was observed in five main flavonoids under the light transmittance 60.3%among the five light treatments,which were Rutin 346.85μg/g,Quercetin 502.75μg/g,Luteolin 299.53μg/g,Kaempferol 241.90μg/g and Isorhamnetin 273.35μg/g,and the photosynthetic efficiency in control group(100%light transmittance)was the best.There was the highest photosynthetic efficiency under full light conditions,and the light transmittance about 60.3%was most conducive to the accumulation of flavonoids during the cultivation of Taraxacum mongolicum,and the light transmittance below 60.3%is adverse to its growth.

关 键 词：蒲公英(Taraxacum mongolicum Hand.-Mazz.) 光照度 光合参数 黄酮类组分 

分 类 号：R931.71[医药卫生—生药学] S647[医药卫生—药学]