硫酸-香草醛法测定地黄总环烯醚萜苷含量  

Determination of total iridoid glutinoside content of Rehmannia glutinosa by Sulfo-Phospho-Vanillin method

作　　者：孙鹏[1] 马军政 段晨晨 付娜娜 黄勇[2] 李先恩[1] SUN Peng;MA Jun-zheng;DUAN Chen-chen;FU Na-na;HUANG Yong;LI Xian-en(Institute of Medicinal Plant Development,Chinese Academy of Medical Science,Beijing 100193,China;Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]中国医学科学院药用植物研究所,北京100193 [2]河南农业大学,郑州450002

出　　处：《湖北农业科学》2021年第5期115-117,共3页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(81373915);国家中药标准化项目(ZYBZH-C-JL-24-03)。

摘　　要：建立地黄(Rehmannia glutinosa)不同部位总环烯醚萜苷的含量测定方法并检测7份地黄种质的总环烯醚萜苷含量。以梓醇为对照品,采用硫酸-香草醛显色法于510 nm对地黄叶片和根中的总环烯醚萜苷含量进行测定。结果表明,该方法在0.01~0.25 mg/mL线性关系良好,回归方程A=1.1917ρ-0.0035,相关系数R2=0.9999。样品平均回收率为100.65%,RSD为1.61%。地黄叶片总环烯醚萜苷的含量普遍高于根含量。试验建立的方法简单快捷、准确,可以用于对地黄根和叶片中总环烯醚萜苷进行含量分析和质量评价。To establish a method for determination of total iridoid glutinoside in the extract of Rehmannia glutinosa and measure the content of total iridoid glutinoside in seven Rehmannia glutinosa germplasms.The Sulfo-Phospho-Vanillin method was applied to compare the content of total iridoid glutinoside in Rehmannia glutinosa.The absorbance was detected at 510 nm.The results showed that a stand curve using catalpol was constructed at a concentration rang of 0.01~0.25 mg/mL.The regression equation was A=1.1917ρ-0.0035,the correlation coefficient is 0.9999.It showed that the content of total iridoid glutinosides in leaves was usually higher than that in roots.The method established in this study is table,simple and rapid for determination of the total iridoid content in Rehmannia glutinosa,and it will provide convenience for content evaluation studies on samples of Rehmannia glutinosa.

关 键 词：地黄(Rehmannia glutinosa) 环烯醚萜苷 种质 

分 类 号：R284.2[医药卫生—中药学]