抑制ERK信号转导通路对磨损颗粒诱导炎性骨溶解的影响  

The Effect of Inhibition of ERK Signaling Pathway on Titanium Particlesinduced Bone Grafting Air Pouch

作　　者：陈德胜[1] 张晨 刘子歌 宋国瑞 郭凤英[3] 李燕[3] CHEN Desheng;ZHANG Chen;LIU Zige;SONG Guorui;GUO Fengying;LI Yan(Department of Orthorpaedics,the General Hospital of Ningxia Medical University,Yinchuan 750004,China;Clinical Medical College,Ningxia Medical University,Yinchuan 750004,China;Basic Medical College,Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学总医院,银川750004 [2]宁夏医科大学临床医学院,银川750004 [3]宁夏医科大学基础医学院,银川750004

出　　处：《宁夏医科大学学报》2021年第2期113-118,共6页Journal of Ningxia Medical University

基　　金：国家自然科学基金(81760405;81760395;81560364);宁夏自然科学基金重点项目(2018AAC02013);宁夏医科大学校级课题重点项目(XZ2018014)。

摘　　要：目的探讨抑制细胞外调节蛋白激酶(ERK)信号转导通路对磨损颗粒诱导骨溶解的影响及可能的机制。方法将45只小鼠随机分为空白对照组、钛颗粒刺激组和钛颗粒+ERK抑制剂组,每组15只。通过HE染色检测植入的小鼠颅骨及气囊壁组织中炎性反应情况;TRAP染色检测植入的小鼠颅骨及气囊壁组织中破骨细胞数目;免疫组化检测植入的小鼠颅骨及气囊壁组织中ERK及肿瘤坏死因子-α(TNF-α)蛋白阳性表达情况。结果HE染色结果:与空白对照组比较钛颗粒刺激组组织标本中存在明显的炎性反应和大量炎性细胞浸润;与钛颗粒刺激组比较,钛颗粒+ERK抑制剂组炎性反应减弱,炎性细胞浸润减少。TRAP染色结果:钛颗粒刺激组阳性细胞数目多于空白对照组,钛颗粒+ERK抑制剂组阳性细胞数目相对于钛颗粒刺激组减少(P<0.05)。免疫组化染色结果:钛颗粒刺激组ERK及TNF-α阳性表达高于空白对照组,钛颗粒+ERK抑制剂组ERK及TNF-α阳性表达相对于钛颗粒刺激组减少(P<0.05)。结论经ERK信号转导通路调控小鼠磨损颗粒诱导的骨溶解中ERK及其下游炎性因子TNF-α活性,从而可能影响破骨细胞的分化和活化,进而调控炎性骨溶解发生、发展。Objective To investigate the effect of ERK signaling pathway inhibition on osteolysis induced by abrasive particles and its possible mechanism.Methods A total of 45 mice were randomly divided into control group(group A),titanium particle stimulation group(group B)and titanium particle+ERK inhibitor group(Group C),with 15 mice in each group.HE staining was used to detect the inflammatory reaction in the implanted mouse skull and the wall of airbag;Trap staining was used to detect the number of osteoclasts in the implanted mouse skull and the wall of airbag;Immunohistochemistry was used to detect the expression of ERK,TNF-αprotein in the implanted mouse skull and the wall of airbag.Results HE staining results showed that compared with the blank control group,there were obvious inflammatory reaction and a large number of inflammatory cell infiltration in tissue samples of the titanium particle stimulation group.Compared with the titanium particle stimulation group,the inflammatory response and infiltration of inflammatory cells in the titanium particle+ERK inhibitor group decreased significantly.TRAP staining results showed that the number of positive cells in the titanium particle stimulation group was significantly higher than that in the blank control group(P<0.05),while the number of positive cells in the titanium particle+ERK inhibitor group was significantly lower than that in the titanium particle stimulation group(P<0.05).Immunohistochemical staining results showed that the positive expression of ERK and TNF-αin the titanium granule stimulation group was significantly higher than that in the blank control group(P<0.05),while the positive expression of ERK and TNF-αin the titanium particle+ERK inhibitor group was significantly lower than that in the titanium granule stimulation group(P<0.05).Conclusion The ERK signaling pathway regulates the activity of ERK and its downstream inflammatory factor TNF-αin osteolysis induced by wear particles in mice,which may affect the differentiation and activation of ost

关 键 词：骨溶解 ERK信号转导通路 PD98059 植骨气囊动物模型 

分 类 号：R683[医药卫生—骨科学]