低胶原降解力蛋白酶用于制革脱毛工序的研究  

Research of enzymatic unhairing using protease and amylase with low collagenase activity

作　　者：宋金枝 王新德 曹珊 李彦春[1] SONG Jinzhi;WANG Xinde;CAO Shan;LI Yanchun(School of Light Industry Science and Engineering,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250353,China)

机构地区：[1]齐鲁工业大学(山东省科学院),轻工科学与工程学院,山东济南250353

出　　处：《中国皮革》2021年第3期39-44,45,共7页China Leather

基　　金：国家自然科学青年基金项目(No.21808170);山东省自然科学基金(ZR2019BB081);国家重点研发计划项目(2017YFB0308401)。

摘　　要：脱毛工序是制革生产中的重大污染源之一。目前常规的脱毛方法为硫化碱脱毛法。硫化物的使用增加了后期废水的处理难度,对水资源造成了严重污染,制约了制革行业的发展。因此,酶脱毛作为一种清洁化脱毛方式在制革行业得到广泛关注。在前期研究中,通过对产酶菌株进行基因改造,获得了对角蛋白具有高降解力同时对胶原具有低降解力的酶。本研究对其酶学性质进行研究,以确定该酶的最佳脱毛条件。研究发现,该酶在pH 10.5及401下对角蛋白的水解能力最强。与此同时,淀粉酶的辅助可以提高毛发去除效率。在WB600-Kerr与淀粉酶用量均为240U/mL的条件下,复合酶液可以成功将毛发全部脱除。The unhairing process is one of the major pollution steps in leather production.The conventional unhairing method is sulfide-lime unhairing.It is difficult to dispose the sulfide-containing wastewater thereby causing serious pollution to water resource,and it seriously limits the development of leather industry.Enzyme unhairing is considered as a clean unhairing method and attracted attention in recent years.In previous study,a new unhairing enzyme was obtained by genetic modification of the enzyme production strains,which possess the high keratinase activity and low collagenase activity.In this research,the properties of WB 600-KerT unhairing enzyme were further studied,and the suitable unhairing condition was determined.It finds that WB 600-KerT has the highest activity at pH 10.5 and 40.Furthermore,the addition of an amylase can improve unhairing effect.The hairs of goat skin can be fully removed,which the activities of WB 600-KerT and amylase are both 240 U/mL.

关 键 词：酶脱毛 角蛋白酶 胶原蛋白酶 淀粉酶 清洁化生产 

分 类 号：TS59[轻工技术与工程—皮革化学与工程]