一种增强型荧光探针检测CN^(-)及在细胞中的应用  

A“turn-on”fluorometric sensor for CN^(-)detection with cell application

作　　者：蔚芳 武旭星 侯杨涛 YU Fang;WU Xu-xing;HOU Yang-tao(Shanxi Institute of Industrial Standardization,Taiyuan 030006,China)

机构地区：[1]山西省工业标准化研究院,山西太原030006

出　　处：《化学研究与应用》2021年第3期580-584,共5页Chemical Research and Application

摘　　要：以甲醇为溶剂,2-羟基-1-萘甲醛和2-氨甲基吡啶1∶1结合生成了一种简单的希夫碱增强型荧光探针(L)。该探针在DMSO∶H_(2)O(3∶2,V/V)的水溶液中加入CN^(-)后,荧光可增强8倍,线性范围为0~120×10^(-5)M,通过核磁滴定证明此作用机理为CN^(-)的去质子化作用。此外,通过荧光共聚焦成像,化合物L可高效选择性地检测人体SiHa癌细胞中的CN^(-)。Methanol,as the solvent,2-hydroxy-1-naphthaldehyde and 2-aminomethylpyridine were combined 1∶1 to form a simple Schiff base enhanced fluorescent probe(L).Adding CN^(-)to the probe in DMSO∶H_(2)O(3∶2,V/V)aqueous solution,the fluorescence could be increased by 8 times,and the linear range was 0~120×10^(-5)M.Partial 1H NMR spectra(600 MHz)of L in DMSO-d6 and the corresponding changes after the sequential addition of cyanide proved that this mechanism was the deprotonation of CN^(-).In addition,through fluorescence microscopic imaging,L could efficiently and selectively detect CN^(-)in human SiHa cancer cells.

关 键 词：希夫碱 荧光探针 CN^(-) 细胞成像 

分 类 号：O623.54[理学—有机化学]