香根草叶片水提液对重金属胁迫下两种草坪草种子萌发的影响  

Effects of Leaf Water Extract of Vetiver Grass on Seed Germination of Two Turfgrass Species under Heavy Metal Stress

作　　者：牛学礼 杨锦玉 钟梦婷 李涵 李可瑶 邹颖 章武 NIU Xue-li;YANG Jin-yu;ZHONG Meng-ting;LI Han;LI Ke-yao;ZOU Ying;ZHANG Wu(School of Life and Science Technology,Lingnan Normal University,Zhanjiang 524048,China;School of Geographical Sciences,Lingnan Normal University,Zhanjiang 524048,China)

机构地区：[1]岭南师范学院生命科学与技术学院,广东湛江524048 [2]岭南师范学院地理科学学院,广东湛江524048

出　　处：《中国草地学报》2021年第3期67-78,共12页Chinese Journal of Grassland

基　　金：岭南师范学院科研处19年校级人才专项(1170919629);广东省自然科学基金项目(2020A151501238);广东省科技创新战略专项资金项目(2018A03030)资助。

摘　　要：为改善重金属胁迫下草坪草种子的萌发,采用室内水分发芽床试验,研究香根草叶片水提液对单一不同浓度重金属(锌、铜、镉)胁迫下高羊茅和多年生黑麦草种子萌发和幼苗生长的影响。结果表明,香根草叶片水提液对高羊茅和多年生黑麦草种子萌发无显著化感作用,但香根草叶片水提液相较对照(蒸馏水)显著提高了Cd^(2+)(200mg/L)胁迫下多年生黑麦草种子的发芽率,Cu^(2+)(100、200mg/L)、Cd^(2+)(100、200mg/L)和Zn^(2+)(200mg/L)胁迫下高羊茅种子的发芽率。香根草叶片水提液显著增加了的Zn^(2+)(600mg/L)、Cu^(2+)(50、100 mg/L)以及Cd^(2+)(50、100、200 mg/L)胁迫下多年生黑麦草的苗长,显著增加了Zn^(2+)(200、400 mg/L)、Cu^(2+)(50 mg/L)以及Cd^(2+)(100、200 mg/L)胁迫下多年生黑麦草的根长和最长根。另外,香根草叶片水提液显著增加了Cd^(2+)(50、200 mg/L)胁迫下高羊茅的苗长,增加了Zn^(2+)(200、400 mg/L)、Cd^(2+)(50、200 mg/L)胁迫下高羊茅的根长和最长根。试验结果为香根草叶片水提液提高高羊茅和多年生黑麦草种子在Cu^(2+)和Cd^(2+)胁迫下的萌发特性提供科学依据,也为香根草更广泛的利用方式提供基础资料。To improve the germination of turfgrass seeds under heavy metal stress,a moisture germination bed test was performed to explore the effects of leaf water extract(LWE)of vetiver grass(Vetiveria zizanioides)on seed germination and seedling growth of tall fescue(Festuca arundinacea)and perennial ryegrass(Lolium perenne)under single stress of zinc(Zn),copper(Cu)and cadmium(Cd)at different concentrations.The results showed that LWE of vetiver grass had no significant allelopathic effect on the germination of tall fescue and perennial ryegrass seeds,but it significantly increased the germination rate of perennial ryegrass seeds under Cd^(2+)(200 mg/L)stress,and the germination rate of tall fescue seeds under Cu^(2+)(100,200 mg/L),Cd^(2+)(100,200 mg/L)and Zn^(2+)(200 mg/L)stress compared to the control(distilled water).LWE of vetiver grass also significantly increased the seedling length of perennial ryegrass under Zn^(2+)(600 mg/L),Cu^(2+)(50 and 100 mg/L)and Cd^(2+)(50,100 and 200 mg/L)stress,and significantly increased the root length and longest root length of perennial ryegrass under Zn^(2+)(200 and 400 mg/L),Cu^(2+)(50 mg/L)and Cd^(2+)(100 and 200 mg/L)stress.In addition,LWE of vetiver grass significantly increased shoot length of tall fescue seedlings under Cd^(2+)(50 and 200 mg/L)stress,and significantly increased the root length and longest root length of tall fescue seedlings under Zn^(2+)(200 and 400 mg/L)and Cd^(2+)(50 and 200 mg/L)stress.In conclusion,this experiment provided a scientific basis for improving the seed germination characteristics of tall fescue and perennial ryegrass by LWE of vetiver grass under Cu^(2+)and Cd^(2+)stress,and also provided basic information for the extensive use of vetiver grass.

关 键 词：香根草 叶片水提液 重金属胁迫 高羊茅 多年生黑麦草 种子萌发 

分 类 号：S634.3[农业科学—蔬菜学]