蒙古冰草根尖高效组培再生体系的建立  

Establishment of Efficient Tissue Culture and Regeneration System of Agropyron mongolicum Keng Root Tip

作　　者：唐芳 徐舶 石凤翎 郑文洋 赵彦 杨紫贻 TANG Fang;XU Bo;SHI Feng-ling;ZHENG Wen-yang;ZHAO Yan;YANG Zi-yi(College of Grassland,Resources and Environment,Inner Mongolia Agricultural University/Key Laboratory of Grassland Resources of Ministry of Education,Hohhot 010011,China)

机构地区：[1]内蒙古农业大学草原与资源环境学院/草地资源教育部重点实验室,内蒙古呼和浩特010011

出　　处：《中国草地学报》2021年第3期113-118,共6页Chinese Journal of Grassland

基　　金：内蒙古农业大学高层次人才引进科研启动项目(NDGCC2016-18);第九批内蒙古自治区“草原英才”。

摘　　要：以蒙古冰草根尖为外植体研究不同激素配比对蒙古冰草愈伤组织诱导及分化的影响,结果表明:蒙古冰草根尖最适愈伤诱导培养基为MS+2,4-D 1.0mg/L+6-BA 0.5mg/L+蔗糖20.0g/L+琼脂7.0g/L,诱导率为73.33%;最适分化培养基为MS+KT 3.0mg/L+NAA 1.0mg/L+蔗糖20.0g/L+琼脂7.0g/L,分化率为70.00%;最适生根培养基为1/2 MS+NAA 0.1mg/L+蔗糖20.0g/L+琼脂7.0g/L,生根率为86.68%。首次建立了以蒙古冰草根尖为外植体的再生体系,再生过程仅70~80d,为蒙古冰草高效遗传转化体系的构建提供了参考。In order to establish an efficient regeneration system in Agropyron mongolicum Keng,we investigated the effects of different hormone ratios on callus induction and differentiation using root tip as explants.The results showed that the optimal medium for callus induction was MS+1.0mg/L 2,4-D+0.5mg/L 6-BA+20.0g/L sucrose+7.0g/L agar,the induction rate was 73.33%;the optimal medium for inducing callus differentiation was MS+3.0mg/L KT+1.0mg/L NAA+20.0g/L sucrose+7.0 g/L agar,the differentiation rate was 70.00%;and the optimal medium for rooting was 1/2 MS+0.1mg/L NAA+20.0g/L sucrose+7.0g/L agar,the rooting rate was 86.68%.In this study,the regeneration system of A.mongolicum Keng with root tip as explants was established for the first time,and the regeneration process was shortened to only 70~80 days.This not only laid the foundation for construction of efficient genetic transformation system in the future,but also provided theoretical support for breeding and cultivar improvement of A.mongolicum Keng.

关 键 词：蒙古冰草 根尖 愈伤组织培养 再生体系 

分 类 号：S543.9[农业科学—作物学]