静原鸡肌苷酸沉积与ADSL基因mRNA表达量相关性分析  

Correl ation Analysis Between Inosine Deposition and ADSL Gene mRNA Expression in Jingyuan Chicken

作　　者：虎红红 黄增文 母童 蔡正云[1] 冯小芳 马正旭 顾亚玲[1] 辛国省[2,3] 国鹏 张娟 Hu Honghong;Huang Zengwen;Mu Tong;Cai Zhengyun;Feng Xiaofang;Ma Zhengxu;Gu Yaling;Xin Guosheng;Guo Peng;Zhang Juan(College of Agriculture,Ningxia University,Yinchuan,750021;School of Life Science,Ningxia University,Yinchuan,750021;Ningxia Feed Engineering Technology Research Center,Yinchuan,750021)

机构地区：[1]宁夏大学农学院,银川7500212 [2]宁夏大学生命科学学院,银川750021 [3]宁夏饲料工程技术研究中心,宁夏银川市750021

出　　处：《基因组学与应用生物学》2020年第12期5442-5448,共7页Genomics and Applied Biology

基　　金：国家自然科学基金项目(31860621)资助。

摘　　要：为研究静原鸡肌苷酸(inosincacid,inosinemonphosphate,IMP)沉积与ADSL mRNA表达的相关性,本研究利用高效液相色谱法对IMP和肌苷含量进行测定;并采用实时荧光定量PCR测定IMP合成过程中关键基因ADSL m RNA表达水平。结果表明,静原鸡公鸡胸肌的IMP含量显著高于腿肌(p<0.05),胸肌的肌苷含量和蒸煮损失率极显著高于腿肌(p<0.01);静原鸡母鸡胸肌IMP和肌苷含量,系水力极显著高于腿肌(p<0.01),腿肌的蒸煮损失率极显著高于胸肌(p<0.01);静原鸡公鸡胸肌、腿肌的IMP与肌苷含量均呈显著负相关(p<0.05);静原鸡母鸡腿肌IMP含量与剪切力呈显著负相关(p<0.05);静原鸡胸肌、腿肌中ADSL mRNA表达量与IMP含量均呈正相关。In order to study the correlation between inosincacid,inosinemonphosphate(IMP)deposition and ADSL mRNA expression,high performance liquid chromatography was used to determine IMP and inosine content.The expression level of ADSL mRNA in IMP synthesis was determined by real-time fluorescence quantitative PCR.The results showed that the IMP content of the chest muscle of Jingyuan cock was significantly higher than that of the leg muscle(p<0.05),the inosine content and cooking loss rate of the chest muscle were extremely significantly higher than those of the leg muscle(p<0.01);The chest muscle IMP,inosine and water-holding capacity of Jingyuan hen were extremely significantly higher than those of leg muscles(p<0.01).The cooking loss rate of leg muscles was extremely significantly higher than that of chest muscles(p<0.01).There was a significant negative correlation of IMP and inosine content in chest muscles and leg muscles of Jingyuan cock(p<0.05);The content of IMP in the leg muscles of Jingyuan hen was significantly negatively correlated with the shearing force(p<0.05).The expression of ADSL mRNA in the chest muscles and leg muscles of Jingyuan chicken were positively correlated with IMP content.

关 键 词：IMP ADSL 静原鸡 实时荧光定量PCR 

分 类 号：S831.2[农业科学—畜牧学]