基于转录组的沟渠豹蛛SSR信息分析  

Analysis of SSR Information in Transcriptome Sequences of Pardosa laura

作　　者：宁青 王立华[1,2] 戴余军 李国元[1] 李长春 Ning Qing;Wang Lihua;Dai Yujun;Li Guoyuan;Li Changchun(School of Life Science and Technology,Hubei Engineering University,Xiaogan,432000;Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables,Xiaogan,432000;Hubei Province Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients,Xiaogan,432000)

机构地区：[1]湖北工程学院生命科学技术学院,孝感432000 [2]特色果蔬质量安全控制湖北省重点实验室,孝感432000 [3]湖北省植物功能成分利用工程技术研究中心,孝感432000

出　　处：《基因组学与应用生物学》2020年第12期5464-5470,共7页Genomics and Applied Biology

基　　金：湖北省自然科学基金面上项目(2017CFB609);区域开发与环境响应湖北省重点实验室(湖北大学)开放基金项目(2016C002);特色果蔬质量安全控制湖北省重点实验室(湖北工程学院)开放基金项目(2017K02)共同资助。

摘　　要：沟渠豹蛛为中国农田广泛分布的游猎型蜘蛛。为评价沟渠豹蛛的基因型多态性,加强害虫天敌的保护与利用,本研究基于沟渠豹蛛转录组数据,挖掘和分析了其SSR位点。结果发现在筛选得到的13366大于1 kb的Unigene序列中有10708个SSR,发生频率为80.12%,重复基元有69种,平均每隔12.13 bp出现一个SSR位点。沟渠豹蛛转录组序列的SSR主要集中在单碱基重复(72.67%)和二碱基重复(20.34%),其中单碱基重复中的优势重复基元为A/T(69.19%),二碱基重复中的优势重复基元为AT/AT(14.18%)。SSR以重复次数为10,且长度为10 bp的短序列为主。研究结果可为沟渠豹蛛遗传多样性分析和遗传图谱构建等提供参考。The wolf spider Pardos a laura is one of the most common species of wandering spiders in agricultural fields of China.In order to acquire genotype polymorphism of P.laura and strengthen the protection of spider as natural enemies of pests,we screened and analyzed its SSR locus based on P.laura transcriptome database.In total,10708 SSRs were detected in 13366 Unigenes more than 1 kb,which was represented by 69 repeat motifs.The frequency of SSRs was detected to be 80.12%,with an average occurrence of per 12.13 bp in length.The mono-nucleotide was the most abundant repeat motif(72.67%),followed by di-nucleotide(20.34%).The dominant repeat motifs in mono-nucleotide and di-nucleotide were A/T(69.19%)and AT/AT(14.18%),respectively.Most of the SSR are less than ten times of repetition and are 10 bp in length.The study could provide references for further research on genetic diversity and construction of genetic map of P.laura.

关 键 词：沟渠豹蛛 转录组 SSR信息分析 

分 类 号：Q953[生物学—动物学]