原核表达家蝇Phormicin蛋白对金黄色葡萄球菌的抑菌活性分析  

Inhibition Activity Analysis of Housefly Phormicin Prokaryotic Expression Protein to S.aureus

作　　者：王兵[1,2,3] 杜兵 韦鹏威 黄伟康 姚杨 伍仕梅 万珊 朱贵明[1] 吴建伟[5] Wang Bing;Du Bing;Wei Pengwei;Huang Weikang;Yao Yang;Wu Shimei;Wan Shan;Zhu Guiming;Wu Jianwei(Key Laboratory of Biology and Medical Engineering,Guizhou Medical University,Guiyang,550025;Engineering Research Center of Medical and Pharmacy Biotechnology,Guizhou Medical University,Guiyang,550025;Department of Biotechnology,Guizhou Medical University,Guiyang,550025;Department of Microbial Immunology,The First Affiliated Hospital of Guizhou Medical University,Guiyang,550025;Department of Human parasites,School of Basic Medical Sciences,Guizhou Medical University,Guiyang,550025)

机构地区：[1]贵州医科大学,贵州医科大学生物与医学工程重点实验室,贵阳550025 [2]贵州医科大学,医药生物技术工程研究中心,资源昆虫研究室,贵阳550025 [3]贵州医科大学,生物技术教研室,贵阳550025 [4]贵州医科大学第一附属医院,微生物免疫科,贵阳550025 [5]贵州医科大学,基础医学院,病原生物学教研室,贵阳550025

出　　处：《基因组学与应用生物学》2020年第12期5533-5539,共7页Genomics and Applied Biology

基　　金：国家自然科学基金(31760250);贵州省科技计划(黔科合基础[2019]1273号);贵州医科大学博士启动基金(Academy-PHD-J-2014-018);贵阳市科技计划(筑科合同[20151001]社15号);贵州省卫健委科学计划(gzwjkj2015-1-028);贵阳市政府-贵州医科大学联合基金(筑科合同[20161001]023)、(筑科合同[2017]5-26号)共同资助。

摘　　要：家蝇Phormicin是一类具有广普抗菌活性的抗菌肽,属于防御素家族。本研究采用原核表达法纯化并获得家蝇Phormicin A、Phormicin B蛋白,将其作用于金黄色葡萄球菌标准株,通过微量液体稀释法测定其最小抑菌浓度分别为0.625 mmol/L和0.469 mmol/L。进一步绘制了二者对金黄色葡萄球菌的时间杀菌曲线,并利用牛津杯法进一步验证了二者的抗菌效果。结果显示,二者对金黄色葡萄球菌都具有一定的抑菌效果。本研究为进一步探索Phormicin在家蝇天然免疫和防御中的机制提供理论参考。Phormicin in Musca domestica is a kind of antimicrobial peptide with broad antibacterial activity,belonging to the defense in family.In this study,Phormicin A and B proteins of housefly were purified by prokaryotic expression method.It was applied to the standard strain of Staphylococcus aureus,and its minimum inhibitory concentration(MIC)was determined to be 0.625 mmol/L and 0.469 mmol/L by trace liquid dilution method.The time growth curve of S.aureus was further drawn.The Oxford cup method was used to verify their antibacterial effect further.These results showed that both of them had certain antibacterial effects on S.aureus.This study provides a theoretical reference for further exploring the mechanism of Phormicin in innate immunity and defense process of housefly.

关 键 词：Phormicin 抗菌肽 家蝇 

分 类 号：R384.2[医药卫生—医学寄生虫学]