根际益生菌挥发性有机物对拟南芥根部基因表达的影响分析  

Effect of B.amyloliquefaciens GB03 Volatile Organic Compounds on Gene Expression of Arabidopsis Roots

作　　者：李菲[1] 李霞 刘杰[1] 张习敏[1] 何小红[1] 乙引[1] Li Fei;Li Xia;Liu Jie;Zhang Ximin;He Xiaohong;Yi Yin(State Forestry Administration,Key Laboratory of Plant Physiology and Developmental Regulation of Guizhou Province,Key Laboratory of Biodiversity Conservation of Southwest Karst Mountains,College of Life Sciences,Guizhou Normal University,Guizhou,550025)

机构地区：[1]贵州师范大学生命科学学院,国家林业局西南喀斯特山地生物多样性保护重点实验室,贵州省植物生理与发育调控重点实验室,贵州550025

出　　处：《基因组学与应用生物学》2020年第12期5549-5557,共9页Genomics and Applied Biology

基　　金：贵州师范大学2017年博士启动基金资助。

摘　　要：细菌挥发性有机物影响植物生理过程的分子机理,是近年研究的热点。研究已经证明,B.amyloliquefaciens GB03挥发性有机物影响拟南芥生长、发育调控和对逆境的响应等多个生理过程。但目前还没有对B.amyloliquefaciens GB03挥发性有机物对拟南芥根部基因表达影响的完整和系统分析。本研究通过高通量转录组测序,筛选出受到根际促生细菌GB03挥发性有机物处理影响的拟南芥功能基因和通路,并对差异表达基因进行GO和KEGG富集分析,获得GB03挥发性有机物影响拟南芥生理过程的分子调控网络。本研究对根际促生细菌挥发性有机物对拟南芥基因表达影响的RNAseq结果与分析进行了报道,此结果对揭示根际促生细菌调控植物生理过程的分子机理,有极大的促进作用。The molecular mechanism of bacterial volatile organic compounds affecting plant physiological processes is a hot topic in recent years.Studies have shown that B.amyloliquefacieus GB03 volatile organic compounds affect Arabidopsis growth,developmental regulation and response to stress and other physiological processes.However,there is no complete and systematic analysis of the effects of B.amyloliquefacienss GB03 volatile organic compounds on Arabidopsis root gene expression.In this study,high-throughput transcriptome sequencing was used to screen Arabidopsis functional genes and pathways affected by volatile organic compounds of rhizosphere plant growth promoting bacteria GB03,and GO and KEGG enrichment analysis was performed on differentially expressed genes.This paper reports on the molecular regulatory network of Arabidopsis physiological processes affected by GB03 volatile organic compounds,which would greatly promotes the understanding of the molecular mechanism of rhizosphere-promoting bacteria regulating plant physiological processes.

关 键 词：根际益生菌 挥发性有机物 拟南芥 转录组 

分 类 号：Q943.2[生物学—植物学]