大刍草SAMS1基因克隆与生物信息学分析  

Cloning and Bioinformatics Analysis of SAMS1 Gene in Balsas Teosinte

作　　者：张玉荣 杨雅舒 申圣圣 王陆军[2] 郭虹霞[2] 邓妍[2] 王创云[2] 杨利艳[1] Zhang Yurong;Yang Yashu;Shen Shengsheng;Wang Lujun;Guo Hongxia;Deng Yan;Wang Chuangyun;Yang Liyan(School of Life Sciences,Shanxi Normal University,Linfen,041004;Institute of Crop Science,Shanxi Academy of Agricultural Sciences,Taiyuan,030031)

机构地区：[1]山西师范大学生命科学学院,临汾041004 [2]山西省农业科学院作物科学研究所,太原030031

出　　处：《基因组学与应用生物学》2020年第12期5660-5668,共9页Genomics and Applied Biology

基　　金：山西省重点研发计划项目(201703D221001-2);院士工作站项目(TYYSZ201707);公益性行业(农业)科研专项(201503124);院农业科技创新研究课题(YCX2018404);三晋学者支持计划专项(2090101)共同资助。

摘　　要：S-腺苷甲硫氨酸合成酶(SAMS)基因催化甲硫氨酸和ATP合成S-腺苷甲硫氨酸,这是目前研究发现的体内S-腺苷甲硫氨酸(SAM)合成的唯一途径,SAMS基因在植物生长发育、新陈代谢以及逆境胁迫响应过程中有着十分重要的作用。本研究对大刍草SAMS1基因(BtSAMS1)进行了克隆,并利用生物信息学工具分析了BtSAMS1蛋白及与其基因序列相似性超过85%的7种其他物种SAMS蛋白。结果显示,BtSAMS1基因全长1185 bp,编码394个氨基酸残基,是位于细胞质中的稳定亲水性蛋白。二级结构主要由无规则卷曲组成,比例大于50%。从三级结构模型来看,蛋白质的结构为四聚体。其他7种蛋白质与BtSAMS1蛋白在理化性质及结构中都存在较高的相似性,表明SAMS蛋白具有高度保守性。S-Adenosylmethionine Synthase(SAMS)gene catalyzes the synthesis of S-Adenosylmethionine with methionine and ATP as substrate,which is the only way to synthesize S-Adenosylmethionine(SAM)in vivo.SAMS plays an important role in plant growth and development,metabolism and stress response.In this study,the SAMS1 gene of Balsas teosinte(BtSAMS1)was cloned,and the BtSAMS1 protein and other SAMS proteins from 7 species,whose sequences are more than 85% similar to BtSAMS,were analyzed with bioinformatics tool.The results showed that BtSAMS1 was 1185 bp,encoding 394 amino acid residues,and the protein was a stable hydrophilic protein located in cytoplasm.Its secondary structure is mainly composed of irregular curls and the proportion is more than 50%.According to the tertiary structure model,the structure of protein is tetramer.The other seven proteins are highly similar to BtSAMS1 proteins in their physical and chemical properties and structures,which indicates that SAMS proteins are highly conserved.

关 键 词：大刍草 SAMS 基因克隆 生物信息学 

分 类 号：Q943.2[生物学—植物学] S513[农业科学—作物学]