A high-strength mineralized collagen bone scaffold for large-sized cranial bone defect repair in sheep  

作　　者：Shuo Wang Zhijun Zhao Yongdong Yang Antonios G.Mikos Zhiye Qiu Tianxi Song Fuzhai Cui XiumeiWang Chunyang Zhang 

机构地区：[1]State Key Laboratory of New Ceramics and Fine Processing,Key Laboratory of Advanced Materials of Ministry of Education,School of Materials Science and Engineering,Tsinghua University,Beijing 100084,China [2]Department of Neurosurgery,The First Affiliated Hospital of Baotou Medical School,Baotou 014010,China [3]Dongzhimen Hospital Affiliated Beijing University of Chinese Medicine,Beijing 100700,China [4]Department of Bioengineering,Rice University,Houston,TX 77030,USA [5]Beijing Allgens Medical Science and Technology Co.,Ltd.,Beijing 100176,China

出　　处：《Regenerative Biomaterials》2018年第5期283-292,共10页再生生物材料（英文版）

基　　金：supported by National Natural Science Foundation of China(No.51572144,81360164 and 81660214);111 Project(No.B17026);Beijing Nova Program(Z161100004916051)。

摘　　要：Large-sized cranial bone defect repair presents a great challenge in the clinic.The ideal cranioplasty materials to realize the functional and cosmetic recovery of the defect must have sufficient mechanical support,excellent biocompatibility,good osseointegration and biodegradability as well.In this study,a high-strength mineralized collagen(MC)bone scaffold was developed with biomimetic composition,microstructure and mechanical properties for the repair of sheep largesized cranial bone defects in comparison with two traditional cranioplasty materials,polymethyl methacrylate and titanium mesh.The compact MC scaffold showed no distinct pore structure and therefore possessed good mechanical properties.The strength and elastic modulus of the scaffold were much higher than those of natural cancellous bone and slightly lower than those of natural compact bone.In vitro cytocompatibility evaluation revealed that the human bone marrow mesenchymal stem cells(hBMSC)had good viability,attachment and proliferation on the compact MC scaffold indicating its excellent biocompatibility.An adult sheep cranial bone defect model was constructed to evaluate the performances of these cranioplasty materials in repairing the cranial bone defects.The results were investigated by gross observation,computed tomography scanning as well as histological assessments.The in vivo evaluations indicated that compact MC scaffold showed notable osteoconductivity and osseointegration with surrounding cranial bone tissues by promoting bone regeneration.Our results suggested that the compact MC scaffold has a promising potential for large-sized cranial bone defect repair.

关 键 词：mineralized collagen cranial bone defect bone regeneration SHEEP cranioplasty materials 

分 类 号：R31[医药卫生—基础医学]