多吡啶双核铜配合物的合成、结构、核酸酶活性及体外细胞毒性  

Synthesis,Structure,Nuclease Activity and Cytotoxicity of Polypyridyl Dinuclear Copper(II)Complex

作　　者：乔佩佩 杜欣 刘广欢 张永坡[1] 高春艳[1] 王敏[2] 岳爱琴[2] 赵晋忠[1] 杜维俊[2] Qiao Peipei;Du Xin;Liu Guanghuan;Zhang Yongpo;Gao Chunyan;Wang Min;Yue Aiqin;Zhao Jinzhong;Du Weijun(Department of Basic Sciences,Shanri Agricultural University,Taigu 030801,China;College of Agronomy,Shanri Agricutural University,Taigu 030801,China)

机构地区：[1]山西农业大学基础部,山西太谷030801 [2]山西农业大学农学院,山西太谷030801

出　　处：《南开大学学报（自然科学版）》2021年第1期1-8,共8页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：山西省重点研发计划(201903D221036);山西省自然科学基金(201701D221157,201701D221158);山西省高等学校大学生创新创业训练项目(2020141)。

摘　　要：合成了一例双核铜配合物[Cu_(2)L(NO_(3))(PhCOO)_(2)(H_(2)O)](ClO_(4))·3H_(2)O(1)(L=4,4'-二(N,N-二(吡啶-2-甲基))氨基二苯甲烷).采用元素分析、红外光谱对配合物进行表征并解析了其单晶结构.单晶结构表明每个Cu中心均为五配位畸变的四方锥构型.利用光谱实验研究了配合物与DNA的结合能力,凝胶电泳实验进一步证明了配合物的化学核酸酶活性,并对切割机理进行了研究.此外MTT实验测定了该配合物对体外HeLa、HepG-2和SGC-7901肿瘤细胞生长的抑制能力.A new dinuclear copper(n)complex[Cu_(2)L(NO_(3))(PhCOO)_(2)(H_(2)O)](ClO_(4))·3H_(2)O,where L is a polypyridyl ligand named 4,4'-methylenebis(N,N-bis(pyridin-2-ylmethyl)aniline),has been synthesized and characterized by elemental analysis and infrared spectroscopy.The single crystal structure shows that each Cu(II)center adopts a tetragonal-pyramidal configuration with five coordination distortions;the combination of the complex and DNA was studied by spectral experiments.Gel electrophoresis experiments further proved the chemical nuclease activity of the complex and the cleavage mechanism was studied.In addition,the M TT assay determined the inhibitory ability of the complex on the growth of HeLa,HepG-2 and SGC-7901 tumor cells in vitro.

关 键 词：铜配合物 荧光淬灭 化学核酸酶活性 抗肿瘤 

分 类 号：O614.121[理学—无机化学]