烯啶虫胺对棉铃虫神经细胞钠、钙通道的影响  

Effects of Nitenpyram on Sodium and Calcium Channels in the Central Neurons of Helicoverpa Armigera

作　　者：刘艺 李清亚 刘晓 杨永川 贺秉军[1] 李长胜 马永强[2] Liu Yi;Li Qingya;Liu Xiao;Yang Yongchuan;He Bingjun;Li Changsheng;Ma Yongqiang(The Key Laboratory of Bioactive Materials of Ministry of Education,College of Life Sciences,Nankai University,Tianjin 300071,China;Department of Applied Chemistry,College of Science,China Agricultural University,Beijing 100193,China)

机构地区：[1]南开大学生命科学学院生物活性材料教育部重点实验室,天津300071 [2]中国农业大学理学院应用化学系,北京100193

出　　处：《南开大学学报（自然科学版）》2021年第1期23-29,共7页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金(31871992,31371974);药物化学生物学国家重点实验室开放基金资助。

摘　　要：利用全细胞膜片钳技术检测了新型杀虫剂烯啶虫胺(Nitenpyram)对急性离体培养的棉铃虫Helicoverpaarmigera(Hübner)中枢神经细胞电压门控钠和钙通道的影响.结果表明:烯啶虫胺对棉铃虫神经元TTX敏感型钠通道和L-型钙通道的峰值电流有显著抑制作用;药物作用后,钠及L-型钙通道的激活曲线均左移,钠通道半数激活电压向超极化方向移动约3 mV,钙通道半数激活电压向超极化方向移动约7 mV,均具有统计学差异;烯啶虫胺对钠和L-型钙通道的失活也有影响,使半数失活电压均向超极化方向移动,钠通道的V_(0.5)偏移约2 mV,钙通道的V_(0.5)偏移约5 mV,均具有显著性差异.另外,烯啶虫胺可显著增大钠通道和L-型钙通道的窗口电流.最终确认烯啶虫胺对棉铃虫幼虫中枢神经元TTX敏感钠通道和L-型钙通道的峰值电流及通道的激活和失活都有影响,使钠、钙通道窗口电流增大,棉铃虫中枢神经细胞的钠、钙通道为烯啶虫胺的潜在作用靶标.The effects of nitentpyram on the voltage-gated sodium and calcium channels in the central neurons of Helicoverpa armigera(Hibner)were examined by the whole-cell patch clamp technique.The results showed that nitenpyram significantly inhibited the peak currents of sodium and L-type calcium chan-nels.Nitenpyram also afected the activation kinetics of sodiurm and calcium channels significantly.After the application of nitenpyram,both the activation curves of sodium and L-type calcium channels shifted nega-tively,the half activation voltage(Vas)of sodium channel shifted toward hyperpolarization direction about 3 mV,and Vas of calcium channels shifted negatively about 7 mV.At the same time,nitenpyram induced negative shift to the inactivation of sodium and L-calcium channels.The Vas shifted toward the hyperpolar-ization direction about 2 mV for sodium channels and about 5 mV for calcium channels.Consistent with the effects of nitenpyram on kinetics of activation and inactivation of sodium and calcium channels,niten-pyram increased the window currents of both sodium and the L-type calcium channels significantly.Niten-pyram could inhibit the peak currents of sodium and calcium channels,shift the V_(0.5)of both activation and inactivation of TTX-sensitive sodium channels and L-type calcium channels,and increased the window currents of both sodium and calcium channels.The data indicates that sodium and calcium channels in cen-tral neurons of Helicoverpa armigera are potential targets for nitenpyram.

关 键 词：棉铃虫 电压门控钠通道 L-型钙通道 烯啶虫胺 

分 类 号：Q965.9[生物学—昆虫学]