桃树枝条皮层响应低温的特异蛋白质2-DE分析  

2-DE Analysis of Cold-response Specific Proteins in Cortex of Branches in Peach Tree

作　　者：李永红[1] 周语潮 田启航 常瑞丰[1] 刘国俭[1] 陈湖[1] 王召元[1] LI Yonghong;ZHOU Yuchao;TIAN Qihang;CHANG Ruifeng;LIU Guojian;CHEN Hu;WANG Zhaoyuan(Changli Fruit Institute,Hebei Academy of Agriculture and Forestry Sciences,Changli 066600,China;Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050051,China)

机构地区：[1]河北省农林科学院昌黎果树研究所,河北昌黎066600 [2]河北省农林科学院,河北石家庄050051

出　　处：《华北农学报》2020年第S01期102-108,共7页Acta Agriculturae Boreali-Sinica

基　　金：河北省农林科学院院青年基金项目(2018020301);河北省农林科学院创新工程项目(2019-3-5-1-02,2019-3-4-4);国家现代桃产业技术体系(CARS-30-Z-02);河北省重点研发计划自筹项目(17226341)。

摘　　要：低温是限制桃树生长、发育、繁殖以及地理分布的一个重要环境因子。明确桃响应低温胁迫的分子调控机制有可能为培育和筛选出相对抗寒的桃品系提供研究基础。以抗寒性较强的秋燕桃为试材,将一年生休眠枝条置于-5(CK),-15,-25℃低温处理48 h,应用双向电泳技术(Two-dimensional electrophoresis,2-DE)测定枝条皮层蛋白质表达变化情况。结果显示:通过质谱鉴定发现,有150个蛋白质点的表达丰度在低温胁迫后发生明显变化,丰度显著上调的有97个,丰度显著下调的有53个,通过蛋白质组数据检索成功地鉴定了其中的20个蛋白,发现这些蛋白质主要参与氨基糖和核苷酸糖代谢、蔗糖和淀粉代谢、氨基酸代谢、MAPK信号通路、苯丙氨酸代谢和DNA的复制等代谢过程。利用RT-PCR验证了Alpha淀粉酶(M5VVU6)、叶绿素a/b结合蛋白(M5WUQ0)和环氧化物水解酶(D8L7V9)蛋白编码的基因,随着低温胁迫的增加而上调表达。研究结果暗示了这些代谢途径和3个特异蛋白可能在桃的抗寒机制中起重要作用。Cold stress is a major environmental factor that limits peach growth,development reproduction and distribution.Clarifying the molecular mechanism of peach in response to low temperature stress could provide the research basis for cultivating and screening relatively cold resistant peach varieties.In cold-tolerant peach cultivar Qiuyan were selected as testing materials.1 year dormant branches were exposed to-5(the control),-15,-25℃under controlled environment chamber for 48 h respectively.Two-dimensional electrophoresis(2-DE)were used to analyze proteomics of cortex of branches under 3 different low temperature treatments.The results showed that a total of 150 differentially expressed proteins were identified by mass spectrometry,97 significantly up-regulated proteins and 53 significantly down-regulated after cold stress.After further analysis of the protein spots by proteome data retrieval,20 proteins related to cold stress were identified,involving amino sugar and nucleotide sugar metabolism,starch and sucrose metabolism,amino acid metabolism,MAPK signaling pathway,phenylalanine metabolism and DNA replication.In addition,Real-time PCR(RT-PCR)indicated that three candidate protein-encoding genes Alpha-amylase(M5VVU6),Chlorophyll a/b binding protein(M5WUQ0)and Epoxide hydrolase 3(D8L7V9)were up-regulated with the increase of cold stress.It suggested that these metabolic pathways and three specific proteins might play an important role in the cold-resistant mechanism in peach.

关 键 词：桃 双向电泳(2-DE) 特异蛋白 抗寒性 

分 类 号：S662.1[农业科学—果树学]